TRANSCRIPTION MAP OF THE B-GENOME COMPONENT OF TOMATO GOLDEN MOSAIC-VIRUS AND COMPARISON WITH A COMPONENT TRANSCRIPTS
TRANSCRIPTION MAP OF THE B-GENOME COMPONENT OF TOMATO GOLDEN MOSAIC-VIRUS AND COMPARISON WITH A COMPONENT TRANSCRIPTS
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DOI：
10.1016/0042-6822(89)90577-1
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发表时间：
1989-12-01
期刊：
影响因子：
3.7
通讯作者：
BISARO, DM
中科院分区：
文献类型：
--
作者：
SUNTER, G;BISARO, DM
在先前的研究中，番茄金色花叶病毒（TGMV）的二分基因组被证明是转录成至少六个多聚腺苷酸化RNA（G。Sunter，W. E.加德纳和d. M. Bisaro，1989，Virology 170，243-250）。其中两个，一个1.3 kb的互补正义和0.9 kb的病毒正义转录本，被映射到该双生病毒的B基因组成分。这里给出的更详细的引物延伸和S1核酸酶保护实验的结果定义了对应于跨越BR 1开放阅读框（ORF）的0.9-kb RNA的单个转录单位的限制。这些数据还表明，互补的正义TGMV RNA比我们早期的研究表明的更复杂。对1.3kb BL 1特异性RNA的分析表明，它实际上是一个具有不同起始位点的不同转录本家族。三个转录物具有5“末端，其定位在DNA B的共同区域附近，并且所有这些起始位点都位于BL 1 ORF的上游。对1.6 kb互补正义AL 1 RNA的类似分析表明，一组复杂的转录物也映射到基因组组分A的类似区域。四个转录物具有5“末端，其定位在共同区域附近，但这些起始位点中只有一个位于AL 1开放阅读框（ORF）的起始密码子的上游。所有记录似乎都没有经过处理。讨论了TGMV基因组这些区域中多个转录物的可能意义，并比较了A和B基因组组分的共同区域近端转录单位。
In a previous study, the bipartite genome of tomato golden mosaic virus (TGMV) was shown to be transcribed into at least six polyadenylated RNAs (G. Sunter, W. E. Gardiner, and d. M. Bisaro, 1989, Virology 170, 243-250). Two of these, a 1.3-kb complementary sense and a 0.9-kb viral sense transcript, were mapped to the B genome component of this geminivirus. The results of more detailed primer extension and S1 nuclease protection experiments presented here define the limits of the single transcription unit corresponding to the 0.9-kb RNA which spans the BR1 open reading frame (ORF). The data also demonstrate that complementary sense TGMV RNAs are more complex than indicated by our earlier studies. Analysis of the 1.3-kb BL1-specific RNA indicates that it is actually a family of distinct transcripts with different start sites. Three transcripts have 5'' ends that map near the common region of DNA B and all of these starts sites lie upstream of the BL1 ORF. Similar analysis of the 1.6-kb complementary sense AL1 RNA indicates that a complex set of transcripts also map to the analogous region of genome component A. Four transcripts have 5'' ends that map near the common region but only one of these start sites is upstream of the initiation codon for the AL1 open reading frame (ORF). None of the transcripts appear to be processed. The possible significance of multile transcripts in these regions of the TGMV genome is discussed, and the common region-proximal transcription units of the A and B genome components are compared.