Epicutaneously sensitized food-induced anaphylaxis is ameliorated with "oral tolerance" to antigen.
Epicutaneously sensitized food-induced anaphylaxis is ameliorated with "oral tolerance" to antigen.
复制标题
皮肤过敏的食物引起的过敏反应可通过对抗原的“口服耐受”得到改善。
DOI：
10.1111/exd.14216
复制
发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Yokozeki H.
中科院分区：
文献类型：
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作者：
Mori Y;Ugajin T;Okada K;Handa Y;Umemoto N;Iijima H;Igawa K;Yokozeki H.
食物过敏是暴露于给定食物后的抗原特异性免疫不良反应。多项临床研究表明，口服免疫疗法（OIT）可有效预防和治疗婴儿和儿童的食物过敏。然而，OIT对表皮致敏食物过敏的有效性仍不清楚。之前，我们建立了一种表皮致敏食物过敏的小鼠模型。在该模型中，观察到全身过敏反应，包括肠道和皮肤症状，如过敏反应。我们以两种方式（致敏前OIT或致敏期间OIT）对该模型进行了OIT治疗，并评估了两种方法的预防效果。致敏前的OIT显着改善致敏皮肤中的肥大细胞脱颗粒，但直肠温度或空肠中的肥大细胞脱颗粒没有降低。然而，在致敏阶段给予OIT显著改善了直肠温度的降低以及皮肤和空肠中肥大细胞的脱粒。致敏前的OIT增加了肠系膜淋巴结（MLN）中的调节性T细胞，但不增加脾脏中的调节性T细胞，与非OIT对照相比，它减少了抗原特异性IgG，但不减少IgE的产生。然而，与非OIT对照组相比，致敏期间OIT导致MLN和脾脏中调节性T细胞的增加更大，并减少抗原特异性IgE和IgG生成。因此，致敏阶段的OIT可有效预防表皮致敏食物过敏。
Food allergy is an antigen‐specific immunological adverse reaction after exposure to a given food. Multiple clinical studies showed that oral immunotherapy (OIT) is effective for the prevention and treatment for food allergy that is developed in infants and children. However, the effectiveness of OIT for epicutaneously sensitized food allergy remains unclear. Previously, we established a mouse model of epicutaneous‐sensitized food allergy. In this model, systemic allergic reaction including intestinal and skin symptoms, such as anaphylaxis, was observed. We treated this model with OIT in two ways (OIT before sensitization or OIT during the sensitization phase) and evaluated the preventive effect of both methods. OIT before sensitization significantly ameliorated mast cell degranulation in sensitized skin, but there was no decrease in rectal temperatures or in mast cell degranulation in the jejunum. However, OIT administered during the sensitization phase significantly ameliorated the decrease in rectal temperature and mast cell degranulation in the skin and jejunum. OIT before sensitization increased the regulatory T cells in mesenteric lymph node (MLN), but not in the spleen, and it reduced antigen‐specific IgG, but not IgE, production compared with the non‐OIT control. However, OIT during sensitization caused a greater increase in regulatory T cells in both the MLN and spleen and reduced antigen‐specific IgE and IgG generation compared with the non‐OIT control group. Thus, OIT during the sensitization phase was effective for the prevention of epicutaneous‐sensitized food allergy.