Surprisingly high correlation between early and late stages in non-two-state protein folding
Surprisingly high correlation between early and late stages in non-two-state protein folding
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DOI：
10.1016/j.jmb.2006.01.072
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发表时间：
2006-04-14
影响因子：
5.6
通讯作者：
Kuwajima, K
中科院分区：
文献类型：
--
作者：
Kamagata, K;Kuwajima, K
我们从文献中收集了非双态蛋白质折叠早期和晚期的定量动力学数据，并研究了这两个阶段的动力学之间的关系。这些阶段的速率常数之间存在令人惊讶的高相关性。对数速率常数的相关系数高达0.97，这不是偶然造成的。我们还研究了这两个阶段的对数速率常数与由残基-残基接触图表示的天然三维结构的关系。对数速率常数和从接触图获得的非局部接触簇的数量之间再次存在令人惊讶的高相关性。由于非局部接触簇的数量代表了天然蛋白质中亚结构的整体排列，因此结果强烈表明了亚结构排列对蛋白质折叠早期和晚期动力学的重要性。(c)2006爱思唯尔有限公司保留所有权利。
We collected quantitative kinetic data on early and late stages of folding in non-two-state proteins from the literature, and studied the relationship between the kinetics of the two stages. There was a surprisingly high correlation between the rate constants of these stages. The correlation coefficient of the logarithmic rate constants was as high as 0.97, which could not be caused by chance. We also studied relationships of the logarithmic rate constants of the two stages with native three-dimensional structures represented by the residue-residue contact map. There were again surprisingly high correlations between the logarithmic rate constants and the number of non-local contact clusters obtained from the contact maps. Because the number of non-local contact clusters represents overall arrangement of substructures in a native protein, the results strongly suggested the importance of the arrangement of the substructures, for the kinetics of both early and late stages of protein folding. (c) 2006 Elsevier Ltd. All rights reserved.