Members of the family Comamonadaceae as primary poly(3-hydroxybutyrate-co-3-hydroxyvalerate)-degrading denitrifiers in activated sludge as revealed by a polyphasic approach
Members of the family Comamonadaceae as primary poly(3-hydroxybutyrate-co-3-hydroxyvalerate)-degrading denitrifiers in activated sludge as revealed by a polyphasic approach
复制标题
DOI：
10.1128/aem.68.7.3206-3214.2002
复制
发表时间：
2002-07-01
影响因子：
4.4
通讯作者：
Hiraishi, A
中科院分区：
文献类型：
--
作者：
Khan, ST;Horiba, Y;Hiraishi, A
采用多相生物标记和分子生物学分析方法，结合培养方法，研究了活性污泥中聚羟基丁酸-3-羟基戊酸共聚物（PHBV）降解菌的分布和系统发育关系。从不同污水处理厂的活性污泥中分离到23株PHBV降解菌。16 S核糖体DNA（rDNA）序列比较表明，20个分离物被确定为科丛毛单胞菌，β-变形菌的主要群体的成员。在实验室规模的反应器中，当来自不同植物的污泥在絮凝条件下用PHBV驯化时，硝酸盐的去除率在前4周内线性增加，并在稳定状态下达到20 mg NO3--N h（-1）g干污泥（-1）。在实验室规模的污泥驯化过程中的细菌群落变化进行了监测rRNA靶向荧光原位杂交和醌分析。这两种方法都表明，在驯化过程中，无论其来源如何，实验室污泥中的β-Proteobaeteria的数量都急剧增加。从两种不同驯化污泥中构建了16 SrDNA克隆文库，并对文库中的37个克隆进行了遗传学分析。大多数16 S rDNA克隆与丛枝藻科的成员聚在一起。我们的多相方法的结果表明，β-Protcobacteria，特别是科丛枝藻科的成员，是活性污泥中的主要PHBV降解微生物。我们的数据提供了有用的信息，以固体生物聚合物作为电子供体的新的脱氮系统的开发。
The distribution and phylogenetic affiliations of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV)-degrading denitrifying bacteria in activated sludge were studied by a polyphasic approach including culture-independent biomarker and molecular analyses as well as cultivation methods. A total of 23 strains of PHBV-degrading denitrifiers were isolated from activated sludges from different sewage treatment plants. 16S ribosomal DNA (rDNA) sequence comparisons showed that 20 of the isolates were identified as members of the family Comamonadaceae, a major group of beta-Proteobacteria. When the sludges from different plants were acclimated with PHBV under denitrifying conditions in laboratory scale reactors, the nitrate removal rate increased linearly during the first 4 weeks and reached 20 mg NO3--N h(-1) g of dry sludge(-1) at the steady state. The bacterial-community change in the laboratory scale sludges during the acclimation was monitored by rRNA-targeted fluorescence in situ hybridization and quinone profiling. Both approaches showed that the population of beta-Proteobaeteria in the laboratory sludges increased sharply during acclimation regardless of their origins. 16S rDNA clone libraries were constructed from two different acclimated sludges, and a total of 37 clones from the libraries were phylogenetically analyzed. Most of the 16S rDNA clones were grouped with members of the family Comamonadaceae. The results of our polyphasic approach indicate that beta-Protcobacteria, especially members of the family Comamonadaceae, are primary PHBV-degrading denitrifiers in activated sludge. Our data provide useful information for the development of a new nitrogen removal system with solid biopolymer as an electron donor.