Topsin-M: the new benomyl for mycorrhizal-suppression experiments
Topsin-M: the new benomyl for mycorrhizal-suppression experiments
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DOI：
10.3852/08-024r
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发表时间：
2008-07
期刊：
影响因子：
2.8
通讯作者：
G. Wilson;M. M. Williamson-M.
中科院分区：
文献类型：
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作者：
G. Wilson;M. M. Williamson-M.
杀菌剂苯菌灵是田间和温室试验中最常用的杀菌剂，在这些试验中需要抑制丛枝菌根真菌(AMF)。不幸的是，苯菌灵已不再生产，因此不能用于实验用途，也没有人建议将杀菌剂作为实验中抑制菌根真菌的成功替代品。在这项研究中，我们在田间和温室实验中考察了杀菌剂topsin M®(Topsin)抑制菌根共生的可能性。托布津减少了AMF在具有真菌营养的暖季牧草仙人掌上的定植，显著减少了植物生物量的生产。托布津减少了兼性真菌营养的冷季型牧草帕斯铜的AMF定植，但没有显著减少生物量的产生。施氮磷肥能够补偿因使用杀菌剂而减少的生物量，因为施了托布津杀菌剂的植株的生物量产量与未施托布津的施肥对照没有显着差异。虽然我们并不提倡顶新杀菌剂是菌根抑制控制的通用机制，但在苯菌灵被发现成功的系统中，顶新似乎是一种有用的、可用的和成功的替代品。
The fungicide benomyl was the most commonly used biocide for both field and greenhouse experiments in which arbuscular mycorrhizal fungal (AMF) suppression is desired. Unfortunately benomyl is no longer manufactured and therefore is not available for experimental use and no fungicide has been proposed as a successful alternative for experimentally suppressing mycorrhizal fungi. In this study we examined the potential for the fungicide Topsin M® (topsin) to suppress mycorrhizal symbiosis in both field and greenhouse experiments. Topsin reduced AMF colonization of the obligately mycotrophic, warm-season grass Andropogon gerardii with a large and significant reduction in plant biomass production. Topsin reduced AMF colonization of the facultatively mycotrophic, cool-season grass Pascopyron smithii but did not significantly reduce biomass production. Fertilization with nitrogen and phosphorus was able to compensate for reductions in biomass due to the application of fungicide because biomass production of plants that received topsin fungicide was not significantly different from fertilized controls not receiving topsin. While we are not advocating that topsin fungicide is a universal mechanism for mycorrhizal-suppressed controls, in systems where benomyl was found to be successful topsin appears to be a useful, available and successful alternative.