In Vitro antibacterial efficacy of 21 Indian timber-yielding plants against multidrug-resistant bacteria causing urinary tract infection.
In Vitro antibacterial efficacy of 21 Indian timber-yielding plants against multidrug-resistant bacteria causing urinary tract infection.
复制标题
DOI：
10.1016/j.phrp.2013.10.007
复制
发表时间：
2013-12
影响因子：
4.3
通讯作者：
Padhy, Rabindra N
中科院分区：
文献类型：
--
作者：
Mishra, Monali P;Padhy, Rabindra N
筛选21种木材生产植物的甲醇叶提取物对从医院临床样品中分离的9种尿路病原菌（粪肠球菌、金黄色葡萄球菌、鲍曼不动杆菌、弗氏柠檬酸杆菌、产气肠杆菌、大肠杆菌、肺炎克雷伯菌、奇异变形杆菌和铜绿假单胞菌）的抗菌活性。通过Kirby-Bauer纸片扩散法对细菌菌株进行抗生素敏感性试验。采用琼脂孔扩散法对9种泌尿系病原菌的多药耐药菌株进行了抗菌活性检测。2株革兰氏阳性菌E. faecalis和S.金黄色葡萄球菌对18种抗生素中的14种有耐药性。革兰氏阴性菌A. baumannii，C. freundii、E. aerogenes、产气肠杆菌E. coli，K.在使用的14种抗生素中，肺炎假单胞菌、奇异假单胞菌和铜绿假单胞菌分别对10、12、9、11、11、10和11种抗生素耐药。尖叶金龟子叶浸提液对黄瓜条斑病菌的最大抑菌圈为29 mm。aureus和28 mm。粪球菌和铜绿假单胞菌。决明子对E.粪肠球菌E.产气假单胞菌和铜绿假单胞菌。根据最低抑菌浓度和最低杀菌浓度，对泌尿道病原菌最有效的10种植物依次为：C. tora> A.尖叶虎尾草>油枝虎尾草>檀香紫檀>洋丁香>凹叶Bridelia retusa>红毛虎尾草>硬籽虎尾草>柚木>花穗虎尾草以下8种植物具有中等的控制能力：菠萝蜜、印楝、宽叶黄檀、柠檬桉、石梓、水黄皮、有袋紫檀和大叶桉。e. coli，其次为A. baumannii，C. freundii、E.产气假单胞菌、奇异假单胞菌和铜绿假单胞菌通过较高量/水平的叶提取物控制。所有植物的植物化学物质进行了定性估计。大多数的木材生产植物的研究，在体外控制能力对MDR尿路病原菌。
To screen methanolic leaf extracts of 21 timber-yielding plants for antibacterial activity against nine species of uropathogenic bacteria isolated from clinical samples of a hospital (Enterococcus faecalis, Staphylococcus aureus, Acinetobacter baumannii, Citrobacter freundii, Enterobacter aerogenes, Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, and Pseudomonas aeruginosa). Bacterial strains were subjected to antibiotic sensitivity tests by the Kirby–Bauer's disc diffusion method. The antibacterial potentiality of leaf extracts was monitored by the agar-well diffusion method with multidrug-resistant (MDR) strains of nine uropathogens. Two Gram-positive isolates, E. faecalis and S. aureus, were resistant to 14 of the 18 antibiotics used. Gram-negative isolates A. baumannii, C. freundii, E. aerogenes, E. coli, K. pneumoniae, P. mirabilis, and P. aeruginosa were resistant to 10, 12, 9, 11, 11, 10, and 11 antibiotics, respectively, of the 14 antibiotics used. Methanolic leaf extracts of Anogeissus acuminata had the maximum zone of inhibition size—29 mm against S. aureus and 28 mm against E. faecalis and P. aeruginosa. Cassia tora had 29 mm as the zone of inhibition size for E. faecalis, E. aerogenes, and P. aeruginosa. Based on the minimum inhibitory concentration and minimum bactericidal concentration values, the most effective 10 plants against uropathogens could be arranged in decreasing order as follows: C. tora > A. acuminata > Schleichera oleosa > Pterocarpus santalinus > Eugenia jambolana > Bridelia retusa > Mimusops elengi > Stereospermum kunthianum > Tectona grandis > Anthocephalus cadamba. The following eight plants had moderate control capacity: Artocarpus heterophyllus, Azadirachta indica, Dalbergia latifolia, Eucalyptus citriodora, Gmelina arborea, Pongamia pinnata, Pterocarpus marsupium, and Shorea robusta. E. coli, followed by A. baumannii, C. freundii, E. aerogenes, P. mirabilis, and P. aeruginosa were controlled by higher amounts/levels of leaf extracts. Phytochemicals of all plants were qualitatively estimated. A majority of timber-yielding plants studied had in vitro control capacity against MDR uropathogenic bacteria.