A signal from inside the peroxisome initiates its division by promoting the remodeling of the peroxisomal membrane
A signal from inside the peroxisome initiates its division by promoting the remodeling of the peroxisomal membrane
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DOI：
10.1083/jcb.200609072
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发表时间：
2007-04-23
影响因子：
7.8
通讯作者：
Titorenko, Vladimir I.
中科院分区：
文献类型：
--
作者：
Guo, Tong;Gregg, Christopher;Titorenko, Vladimir I.
我们定义了服务过氧化物酶体分裂的蛋白质和脂质团队的空间和时间重组的动态。过氧化物酶体只有在获得参与脂质代谢的全套基质蛋白后才具有分裂能力。基质蛋白使过氧化物酶体超载，会促进酰基辅酶 A 氧化酶 (Aox)（一种脂肪酸 β 氧化酶）从基质重新定位到膜上。 Aox 与 Pex16p（一种过氧化物酶体生物发生所需的膜相关过氧化物蛋白）的结合启动膜中磷脂酸和二酰甘油 (DAG) 的生物合成。这两种脂质的形成以及随后的 DAG 跨双层运动启动了过氧化物酶 Pex10p 和 Pex19p、动力样 GTPase Vps1p 以及过氧化物酶体表面上几种肌动蛋白细胞骨架蛋白之间复合物的组装。该蛋白质组促进膜裂变，从而执行过氧化物酶体分裂的最终步骤。
We define the dynamics of spatial and temporal reorganization of the team of proteins and lipids serving peroxisome division. The peroxisome becomes competent for division only after it acquires the complete set of matrix proteins involved in lipid metabolism. Overloading the peroxisome with matrix proteins promotes the relocation of acyl-CoA oxidase (Aox), an enzyme of fatty acid beta-oxidation, from the matrix to the membrane. The binding of Aox to Pex16p, a membrane-associated peroxin required for peroxisome biogenesis, initiates the biosynthesis of phosphatidic acid and diacylglycerol (DAG) in the membrane. The formation of these two lipids and the subsequent transbilayer movement of DAG initiate the assembly of a complex between the peroxins Pex10p and Pex19p, the dynamin-like GTPase Vps1p, and several actin cytoskeletal proteins on the peroxisomal surface. This protein team promotes membrane fission, thereby executing the terminal step of peroxisome division.