The effect of octreotide on wound healing: an in vitro and in vivo experimental study.
The effect of octreotide on wound healing: an in vitro and in vivo experimental study.
复制标题
奥曲肽对伤口愈合的影响：体外和体内实验研究。
DOI：
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发表时间：
1997
期刊：
影响因子：
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通讯作者：
S. Kyzer
中科院分区：
文献类型：
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作者：
D. Alper;A. Levy;A. Kadouri;J. Abarbanel;C. Chaimoff;S. Kyzer
目的
观察奥曲肽对创面愈合的影响。
设计
体外和大鼠实验研究。
设置
以色列教学医院。
材料
培养的人二倍体胎儿成纤维细胞和36只雄性Wistar大鼠。
干预措施
将奥曲肽以2、10、30、60和120 ng/ml的剂量加入成纤维细胞培养物中，并在2、4和6天后对成纤维细胞计数。用36只大鼠制作肠梗阻模型。奥曲肽组（n = 18）皮下注射奥曲肽0.25 μ g/kg，每日2次，分别于第3、7、14天处死6只。对照组注射生理盐水。在三个时间点测量吻合口破裂压和羟脯氨酸含量。
主要观察指标
成纤维细胞计数、吻合口破裂压和羟脯氨酸浓度。
结果
奥曲肽在任何剂量下在任何时间段均不抑制成纤维细胞增殖。奥曲肽组在每个时间点的吻合口破裂压略高，但不显著，奥曲肽组和对照组之间的羟脯氨酸含量无差异。奥曲肽在体外和体内均不抑制伤口愈合。
OBJECTIVE
To investigate the effect of octreotide on wound healing.
DESIGN
Experimental studies in vitro and in rats.
SETTING
Teaching hospital, Israel.
MATERIAL
Cultured human diploid fetal fibroblasts, and 36 male Wistar rats.
INTERVENTIONS
Octreotide was added to cultures of fibroblasts in doses of 2, 10, 30, 60 and 120 ng/ml and fibroblasts were counted after 2, 4, and 6 days. Intestinal anastomoses were made in 36 rats. Rats in the octreotide group (n = 18) were given subcutaneous injections of 0.25 microg/kg twice daily and 6 rats were killed at 3, 7, and 14 days. The control group were given injections of saline. Anastomotic bursting pressures and hydroxyproline content were measured at each of the three times.
MAIN OUTCOME MEASURES
Fibroblast counts, anastomotic bursting pressures, and hydroxyproline concentrations.
RESULTS
Octreotide did not inhibit fibroblast proliferation in any of the doses at any of the time periods. The anastomotic bursting pressure was slightly higher in the octreotide group at each of the time points, but not significantly so, and there was no difference in hydroxyproline content between the octreotide and control groups. Octreotide did not inhibit wound healing either in vitro or in vivo.