Bionic Engineered Protein Coating Boosting Anti‐Biofouling in Complex Biological Fluids
Bionic Engineered Protein Coating Boosting Anti‐Biofouling in Complex Biological Fluids
复制标题
仿生工程蛋白质涂层可增强复杂生物流体的抗生物污损性
DOI：
10.1002/adma.202208824
复制
发表时间：
2022
影响因子：
29.4
通讯作者：
Hongbo Zeng
中科院分区：
文献类型：
--
作者：
Ziqian Zhao;Mingfei Pan;Chenyu Qiao;Li Xiang;Xiong Liu;Wenshuai Yang;Xing;Hongbo Zeng
Implantable medical devices have been widely applied in diagnostics, therapeutics, organ restoration, and other biomedical areas, but often suffer from dysfunction and infections due to irreversible biofouling. Inspired by the self‐defensive “vine‐thorn” structure of climbing thorny plants, a zwitterion‐conjugated protein is engineered via grafting sulfobetaine methacrylate (SBMA) segments on native bovine serum albumin (BSA) protein molecules for surface coating and antifouling applications in complex biological fluids. Unlike traditional synthetic polymers of which the coating operation requires arduous surface pretreatments, the engineered protein BSA@PSBMA (PolySBMA conjugated BSA) can achieve facile and surface‐independent coating on various substrates through a simple dipping/spraying method. Interfacial molecular force measurements and adsorption tests demonstrate that the substrate‐foulant attraction is significantly suppressed due to strong interfacial hydration and steric repulsion of the bionic structure of BSA@PSBMA, enabling coating surfaces to exhibit superior resistance to biofouling for a broad spectrum of species including proteins, metabolites, cells, and biofluids under various biological conditions. This work provides an innovative paradigm of using native proteins to generate engineered proteins with extraordinary antifouling capability and desired surface properties for bioengineering applications.
