Which phenotypic traits of Dictyostelium discoideum farmers are conferred by their bacterial symbionts?
Which phenotypic traits of Dictyostelium discoideum farmers are conferred by their bacterial symbionts?
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DOI：
10.1007/s13199-015-0352-0
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发表时间：
2016-03-01
期刊：
影响因子：
2.5
通讯作者：
Strassmann, Joan E.
中科院分区：
文献类型：
--
作者：
Brock, Debra A.;Jones, Kai;Strassmann, Joan E.
与真核生物共生关系中的细菌伙伴可以对宿主的表型特征产生强大的影响。在这里，我们使用一个简单的真核生物模型-盘基网柄菌及其兼性细菌共生体来探索这个问题。社会变形虫的一些克隆被称为农民，与某些种类的细菌保持共生关系，而另一些克隆被称为非农民，则不能。携带伯克霍尔德氏菌属细菌共生体的盘状D菌农民克隆具有与农业共生相关的四个明显特征：i)段塞迁移距离短，ii)共生体运输，iii)谨慎地收获细菌，以及iv)与农民相关的细菌对毒性的抵抗力。这些特征，连同它们的优势和劣势，可能是由细菌共生体赋予的，也可能是细菌定居的阿米巴所固有的。我们比较了五名农民和五名非农民，无论有没有农民相关的细菌，以区分细菌与宿主内在的直接影响。我们发现，短迁移距离和共生体运输是细菌赋予的特征。谨慎的收获似乎代表了一种受细菌影响但并不完全受细菌控制的特征。对农民相关的伯克霍尔德氏菌的抗性是存在的，无论农民克隆人目前是否携带这种病毒，或者是否已经治愈和新暴露。综上所述，这些数据表明，寄主农民和伯克霍尔德里亚之间的联系并不是最近的。
The bacterial partners in symbiotic relationships with eukaryotes can have a powerful effect on the phenotypic traits of the host. Here we explore this issue using a simple model eukaryote, Dictyostelium discoideum, and its facultative bacterial symbionts. Some clones of the social amoeba D. discoideum, called farmers, maintain symbiotic relationships with certain species of bacteria while other clones, called non-farmers, do not. D. discoideum farmer clones that carry bacterial symbionts in the genus Burkholderia have four distinct traits associated with the farming symbiosis: i) short slug migration distances, ii) symbiont transport, iii) prudent harvesting of bacteria, and iv) resistance to toxicity of the farmer-associated bacteria. These traits, with their advantages and disadvantages, could be either conferred by bacterial symbionts, or intrinsic to the amoebae that have been colonized by bacteria. We compared five farmers and five non-farmers, both with and without farmer-associated bacteria, to disentangle the direct effects of bacteria from those intrinsic to the host. We found that short migration distance and symbiont transport are bacterially conferred traits. Prudent harvesting seems to represent a trait influenced by bacteria but not entirely controlled by them. Resistance to farmer-associated Burkholderia is present whether or not the farmer clone is currently carrying it or has been cured and newly exposed. Taken together, these data suggest that the association between host farmer and Burkholderia is not recent.