Exudative epidermitis in pigs caused by toxigenic Staphylococcus chromogenes
Exudative epidermitis in pigs caused by toxigenic Staphylococcus chromogenes
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DOI：
10.1016/j.vetmic.2004.12.006
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发表时间：
2005-02-25
影响因子：
3.3
通讯作者：
Bille-Hansen, V
中科院分区：
文献类型：
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作者：
Andresen, LO;Ahrens, P;Bille-Hansen, V
产色葡萄球菌与猪葡萄球菌密切相关，猪葡萄球菌被认为是猪渗出性表皮炎（EE）的病原体。产色链球菌是猪、牛和家禽正常皮肤菌群的一部分，迄今为止被认为对猪无致病性。通过使用对猪链球菌剥脱性毒素具有特异性的多重 PCR 鉴定出产生 B 型剥脱性毒素的产色链球菌菌株 ExhB。在免疫印迹分析中，产色链球菌的脱落毒素与对猪链球菌 ExhB 具有特异性的多克隆和单克隆抗体发生反应，表观分子量为 30 kDa。对编码产色链球菌脱落毒素的基因进行测序表明，带有信号肽的毒素和成熟毒素的分子量分别为 30,553 和 26,694 Da。来自产色链球菌菌株 VA654 和 S. hyicus 菌株 1289D-88 的 exhB 基因的比较显示出 DNA 序列的七个碱基对和推导的氨基酸序列中的两个氨基酸残基的差异。猪实验性地接种了产色链球菌菌株 VA654。通过临床观察和皮肤变化的组织病理学评估，所有猪都显示出全身渗出性表皮炎的发展。从猪体内未分离到产生毒素的猪链球菌，所有 ExhB 阳性细菌分离株均被鉴定为产色链球菌。这证实了致病因子是接种的产色链球菌菌株VA654。本研究结果表明，产色链球菌可能引起猪的渗出性表皮炎。 (C) 2004 Elsevier B.V. 保留所有权利。
Staphylococcus chromogenes is closely related to Staphylococcus hyicus, which is recognised as the causative agent of exudative epidermitis (EE) in pigs. S. chromogenes is part of the normal skin flora of pigs, cattle and poultry and has so far been considered non-pathogenic to pigs. A strain of S. chromogenes producing exfoliative toxin type B, ExhB, was identified by the use of a multiplex PCR specific for the exfoliative toxins from S. hyicus. The exfoliative toxin from S. chromogenes reacted in immunoblot analysis with polyclonal and monoclonal antibodies specific to ExhB from S. hyicus and had an apparent molecular weight of 30 kDa. Sequencing the gene encoding the exfoliative toxin from S. chromogenes revealed that the molecular weight of the toxin with the signal peptide and the mature toxin was 30,553 and 26,694 Da, respectively. Comparison of the exhB genes from S. chromogenes strain VA654 and S. hyicus strain 1289D-88 showed differences in seven base pairs of the DNA sequences and in two amino acid residues in the deduced amino acid sequences. Pigs were experimentally inoculated with S. chromogenes strain VA654. By clinical observations and histopathological evaluation of the skin alterations, all pigs revealed development of generalized exudative epidermitis. No toxin producing S. hyicus was isolated from the pigs and all ExhB-positive bacterial isolates were identified as S. chromogenes. This confirmed that the disease-causing agent was the inoculated S. chromogenes strain VA654. The results of this study show that S. chromogenes may cause exudative epidermitis in pigs. (C) 2004 Elsevier B.V. All rights reserved.