Effects of concentrated growth factor on proliferation, migration, and differentiation of human dental pulp stem cells in vitro
Effects of concentrated growth factor on proliferation, migration, and differentiation of human dental pulp stem cells in vitro
复制标题
浓缩生长因子对人牙髓干细胞体外增殖、迁移和分化的影响。
DOI：
10.1177/2041731418817505
复制
发表时间：
2018-12-21
影响因子：
8.2
通讯作者：
Chen, Zhi
中科院分区：
文献类型：
--
作者：
Jin, Runze;Song, Guangtai;Chen, Zhi
浓缩生长因子是一种新型的自体血浆提取物，含有多种促进组织再生的生长因子。本研究旨在探讨浓缩生长因子对人牙髓干细胞的生物学效应。通过扫描电镜观察浓缩生长因子支架的微观结构和生物相容性。将牙髓干细胞暴露于不同浓度（5%、10%、20%、50%或80%）的浓缩生长因子提取物后，评估细胞增殖和迁移、成牙本质细胞和内皮细胞分化潜能。结果表明，浓缩生长因子支架具有与血小板和白细胞连接的多孔纤维蛋白网络，与牙髓干细胞具有良好的生物相容性。在浓缩生长因子处理组中以剂量依赖性方式检测到更高的细胞增殖率。有趣的是，与对照组相比，低剂量（
Concentrated growth factor, a novel autologous plasma extract, contained various growth factors which promoted tissue regeneration. In this study, we aimed to investigate the biological effects of concentrated growth factor on human dental pulp stem cells. The microstructure and biocompatibility of concentrated growth factor scaffolds were evaluated by scanning electron microscopy. Cell proliferation and migration, odontoblastic and endothelial cell differentiation potential were assessed after exposing dental pulp stem cells to different concentrations (5%, 10%, 20%, 50%, or 80%) of concentrated growth factor extracts. The results revealed that concentrated growth factor scaffolds possessed porous fibrin network with platelets and leukocytes, and showed great biocompatibility with dental pulp stem cells. Higher cell proliferation rates were detected in the concentrated growth factor-treated groups in a dose-dependent manner. Interestingly, in comparison to the controls, the low doses (