Sox30 initiates transcription of haploid genes during late meiosis and spermiogenesis in mouse testes
Sox30 initiates transcription of haploid genes during late meiosis and spermiogenesis in mouse testes
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Sox30 在小鼠睾丸减数分裂晚期和精子发生过程中启动单倍体基因的转录
DOI：
10.1242/dev.164855
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发表时间：
2018-07-01
期刊：
影响因子：
4.6
通讯作者：
Ye, Lan
中科院分区：
文献类型：
--
作者：
Bai, Shun;Fu, Kaiqiang;Ye, Lan
Sox蛋白家族的转录因子包含一个dna结合HMG盒子，是祖细胞命运的关键调节因子。在这里，我们报道了Sox30的表达仅限于减数分裂精母细胞和减数分裂后单倍体。Sox30突变体的雄性不育是由于在早期圆形精子阶段生精停止。具体来说，在缺乏Sox30的情况下，顶体前囊泡不能形成单个顶体细胞器，精子在第2-3步停止。尽管大多数Sox30突变的精母细胞在减数分裂中进展，但二倍体精母细胞的积累表明从减数分裂到减数分裂后阶段的过渡延迟或受损。对分离的阶段特异性生精细胞的转录组分析表明，Sox30控制着减数分裂后核心基因表达程序，该程序早在减数分裂细胞后期就开始了。ChIP-seq分析显示，Sox30与小鼠睾丸中的特定DNA序列结合，其基因组占用率与Tnp1、Hils1、Ccdc54和Tsks等减数分裂后基因的表达呈正相关。这些结果确定Sox30是一个关键的转录因子，控制着从减数分裂晚期到减数分裂后基因表达程序的转变和随后的圆形精子发育。摘要：利用ChIP-seq、减数分裂分析和RNA-seq对Sox30突变体进行分析，确定了Sox30的直接靶点，揭示了Sox30是小鼠睾丸减数分裂后基因的转录因子。
ABSTRACT Transcription factors of the Sox protein family contain a DNA-binding HMG box and are key regulators of progenitor cell fate. Here, we report that expression of Sox30 is restricted to meiotic spermatocytes and postmeiotic haploids. Sox30 mutant males are sterile owing to spermiogenic arrest at the early round spermatid stage. Specifically, in the absence of Sox30, proacrosomic vesicles fail to form a single acrosomal organelle, and spermatids arrest at step 2-3. Although most Sox30 mutant spermatocytes progress through meiosis, accumulation of diplotene spermatocytes indicates a delayed or impaired transition from meiotic to postmeiotic stages. Transcriptome analysis of isolated stage-specific spermatogenic cells reveals that Sox30 controls a core postmeiotic gene expression program that initiates as early as the late meiotic cell stage. ChIP-seq analysis shows that Sox30 binds to specific DNA sequences in mouse testes, and its genomic occupancy correlates positively with expression of many postmeiotic genes including Tnp1, Hils1, Ccdc54 and Tsks. These results define Sox30 as a crucial transcription factor that controls the transition from a late meiotic to a postmeiotic gene expression program and subsequent round spermatid development. Summary: Analysis of Sox30 mutants using ChIP-seq, meiotic analysis and RNA-seq identifies the direct targets of Sox30 and reveals that Sox30 is a transcription factor of postmeiotic genes in mouse testes.