Association of a phloem-limited bacterium with yellow vine disease in cucurbits
Association of a phloem-limited bacterium with yellow vine disease in cucurbits
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DOI：
10.1094/pdis.1998.82.5.512
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发表时间：
1998-05-01
期刊：
影响因子：
4.5
通讯作者：
Sittertz-Bhatkar, H
中科院分区：
文献类型：
--
作者：
Bruton, BD;Fletcher, J;Sittertz-Bhatkar, H
自1991年以来，在得克萨斯州中部和俄克拉荷马州的葫芦科植物的一种新疾病，指定为黄藤，导致西瓜，哈密瓜的下降和植物死亡。南瓜和南瓜受影响的植物的特征是叶片变黄，韧皮部变色，植物崩溃。发病率的年与年之间的变化范围从零星爆发到早期种植西瓜田的完全作物损失。一个系统的调查，以确定病原体的疾病，包括病原体分离尝试，传播试验，血清学测定与各种抗血清（酶联免疫吸附试验和蛋白质印迹），和DNA杂交与选定的探针（斑点和Southern印迹）。这些测试都没有揭示出黄藤症状的表达与植物中特定微生物或病毒的存在之间的一致关系。然而，透射电子显微镜检查表明，在有症状的植物的韧皮部筛元素的细菌的一致存在。杆状细菌仅在有症状的葫芦中观察到，宽0.25至0.5 μ m，长1.0至3.0 μ m，被三层细胞包膜包围。
Since 1991, a new disease of cucurbits in central Texas and Oklahoma, designated yellow vine, has resulted in the decline and plant death of watermelon, cantaloupe. squash, and pumpkin. Affected plants are characterized by leaf yellowing, phloem discoloration, and plant collapse. Year-to-year variation in disease incidence has ranged from spotty outbreaks to complete crop loss in early-planted watermelon fields. A systematic investigation to determine the causal agent of the disease included pathogen isolation attempts, transmission tests, serological assays with various antisera (enzyme-linked immunosorbent assay and Western blotting), and DNA hybridizations with selected probes (dot and Southern blots). None of these tests revealed a consistent relationship between the expression of yellow vine symptoms and the presence of a particular microorganism or virus in the plant. However, transmission electron microscopic examination showed the consistent presence of a bacterium in the phloem sieve elements of symptomatic plants. The rod-shaped bacteria, observed only in symptomatic cucurbits, measured 0.25 to 0.5 mu m in width and 1.0 to 3.0 mu m in length and were surrounded by a triple-layered cell envelope.