The cytotoxicity of cadmium-based quantum dots
The cytotoxicity of cadmium-based quantum dots
复制标题
镉基量子点的细胞毒性
DOI：
10.1016/j.biomaterials.2011.10.070
复制
发表时间：
2012-02-01
期刊：
影响因子：
14
通讯作者：
Fan, Chunhai
中科院分区：
文献类型：
--
作者：
Chen, Nan;He, Yao;Fan, Chunhai
半导体量子点由于其上级光学性质和在生物医学领域的广泛应用而受到人们的广泛关注。最近，人们对量子点的细胞毒性评估产生了强烈的关注。大多数QD由重金属离子（例如，Cd 2+），这可能会导致潜在的体外毒性，阻碍其实际应用。本文就II-IV类量子点细胞毒性机制的研究进展作一综述。我们研究了一系列水相合成的量子点（aqQDs），即CdTe、CdTe/CdS核壳结构和CdTe/CdS/ZnS核壳结构的aqQDs的细胞毒性。我们的研究结果表明，释放的镉离子负责所观察到的镉基量子点的细胞毒性。CdTe/CdS/ZnS核-壳-壳结构量子点对细胞几乎无毒的事实进一步证实了释放的镉离子对细胞毒性的作用，以及ZnS壳的有效保护。然而，Cd 2+离子的细胞内水平不能是唯一的原因，因为与CdCl 2处理的细胞的比较表明，还有其他因素促成了aqQD的细胞毒性。我们的全基因组基因表达谱和亚细胞定位的aqQDs与同步加速器扫描透射X射线显微镜（STXM）的研究进一步表明，碲化镉量子点的细胞毒性不仅来自镉离子的释放，但也在细胞内分布的量子点纳米粒子和相关的纳米尺度效应。(C)2011爱思唯尔有限公司保留所有权利。
Semiconductor Quantum dots (QDs) have raised great attention because of their superior optical properties and wide utilization in biological and biomedical studies. More recently, there have been intense concerns on cytotoxicity assessment of QDs. Most QDs are made of heavy metal ions (e.g., Cd2+), which may result in potential in vitro toxicity that hampers their practical applications. In this article, we aim to summarize recent progress on mechanistic studies of cytotoxicity of II-IV QDs. We have studied the cytotoxicity of a series of aqueous synthesized QDs (aqQDs), i.e. CdTe, CdTe/CdS core-shell structured and CdTe/CdS/ZnS core-shell-shell structured aqQDs. Our results suggested that released cadmium ions are responsible for the observed cytotoxicity of cadmium-based QDs. The fact that CdTe/CdS/ZnS core-shell-shell structured QDs are nearly nontoxic to cells further confirmed the role of released cadmium ions on cytotoxicity, and the effective protection of the ZnS shell. However, intracellular level of Cd2+ ions cannot be the only reason since the comparison with CdCl2-treated cells suggests there are other factors contributed to the cytotoxicity of aqQDs. Our studies on genome-wide gene expression profiling and subcellular localization of aqQDs with synchrotron-based scanning transmission X-ray microscopy (STXM) further suggest that the cytotoxicity of CdTe QDs not only comes from the release of Cd2+ ions but also intracellular distribution of QD nanoparticles in cells and the associated nanoscale effects. (C) 2011 Elsevier Ltd. All rights reserved.