Nicotine Reduces Human Brain Microvascular Endothelial Cell Response to Escherichia coli K1 Infection by Inhibiting Autophagy.
Nicotine Reduces Human Brain Microvascular Endothelial Cell Response to Escherichia coli K1 Infection by Inhibiting Autophagy.
复制标题
尼古丁通过抑制自噬降低人脑微血管内皮细胞对大肠杆菌 K1 感染的反应
DOI：
10.3389/fcimb.2020.00484
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发表时间：
2020
影响因子：
5.7
通讯作者：
Wang L
中科院分区：
文献类型：
--
作者：
Wu C;Yang M;Liu R;Hu H;Ji L;Zhang X;Huang S;Wang L
研究表明，对环境烟雾的暴露会增加细菌脑膜炎的风险，而尼古丁是环境烟草的核心组成部分。大肠杆菌侵入人类大脑microvascular endothelial cells (HBMECs), the main component of the blood–brain barrier, is not clear yet. Our study found that the increase of HBMEC autophagy level during E. coli K1 infection could decrease the survival of intracellular bacteria, while nicotine exposure could inhibit the HBMEC autophagic response of E. coli K1 infection by activating the NF-kappa B and PI3K/Akt/mTOR pathway. We concluded that nicotine could inhibit HBMEC自噬在大肠杆菌K1感染时自噬，并降低了对大肠杆菌K1的清除作用，从而促进了新生儿脑膜炎的发生和发育。
Studies have shown that exposure to environmental tobacco smoke can increase the risk of bacterial meningitis, and nicotine is the core component of environmental tobacco smoke. Autophagy is an important way for host cells to eliminate invasive pathogens and resist infection. Escherichia coli K1 strain (E. coli K1) is the most common Gram-negative bacterial pathogen that causes neonatal meningitis. The mechanism of nicotine promoting E. coli K1 to invade human brain microvascular endothelial cells (HBMECs), the main component of the blood–brain barrier, is not clear yet. Our study found that the increase of HBMEC autophagy level during E. coli K1 infection could decrease the survival of intracellular bacteria, while nicotine exposure could inhibit the HBMEC autophagic response of E. coli K1 infection by activating the NF-kappa B and PI3K/Akt/mTOR pathway. We concluded that nicotine could inhibit HBMEC autophagy upon E. coli K1 infection and decrease the scavenging effect on E. coli K1, thus promoting the occurrence and development of neonatal meningitis.