Identification of microRNAs response to high light and salinity that involved in beta-carotene accumulation in microalga Dunaliella salina
Identification of microRNAs response to high light and salinity that involved in beta-carotene accumulation in microalga Dunaliella salina
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DOI：
10.1016/j.algal.2020.101925
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发表时间：
2020-06
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影响因子：
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通讯作者：
Sulin Lou;X. Zhu;Zhiyong Zeng;Huina Wang;Jia Bin;Hui Li;Zhangli Hu
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作者：
Sulin Lou;X. Zhu;Zhiyong Zeng;Huina Wang;Jia Bin;Hui Li;Zhangli Hu
MicroRNAs(MiRNAs)是一类内源性非编码RNAs，不仅在动物和陆地植物中，而且在微藻中，都在转录后基因调控中发挥重要作用。在所有的微藻中，杜氏盐藻(D.Salina)的β-胡萝卜素含量最高，最近公布了该物种的基因组草图。到目前为止，还没有几个。已经确定了SalinamiRNAs。在这项研究中，我们进行了深度测序来鉴定野生型的miRNAs。盐藻(对照)和高光高盐处理组(HLS)。分别为对照和HLS构建了3个小RNA(SRNA)文库，分别获得了11,542,169和16,877,598个干净标签。SRNA的长度分布表明，在对照和HLS中，21个核苷酸的sRNA是最丰富的一类，这与模式生物莱茵衣藻的sRNA分布一致。Control中发现了84个已知的miRNAs和1054个新的miRNAs。其中，9个已知的miRNAs和39个新的miRNAs在HLS处理后水平发生了变化。通过qRT-PCR验证了所选择的miRNAs及其靶基因在对照和HLS中的表达差异，结果表明新型m0533-3p在HLS中表达上调，其预测的目标苹果酸脱氢酶编码基因在转录和蛋白质水平上都明显下调。此外，一些关键的类胡萝卜素途径基因，如PSY、PDSandLCYB等都在HLS中表达上调。这些发现为miRNA介导的β-胡萝卜素积累机制Ind提供了新的见解。萨利纳。
MicroRNAs (miRNAs) are a class of endogenous non-coding RNAs that play a crucial role in post-transcriptional gene regulation not only in animals and land plants, but also in microalgae. Among all the microalga, Dunaliella salina (D. salina) has the highest content of beta-carotene, and a draft genome of this species was recently published. Thus far, fewD. salinamiRNAs have been identified. In this study, deep sequencing was performed to identify miRNAs in wild-typeD. salina(Control) and in a high light and high salinity treatment group (HLS). Three small RNA (sRNA) libraries were constructed for Control and HLS, and an average of 11,542,169 and 16,877,598 clean tags were obtained, respectively. The sRNA length distribution showed that 21 nt sRNAs were the most abundant class in both Control and HLS, which is consistent with the sRNA distribution of model organism Chlamydomonas reinhardtii. Eighty-four known miRNAs and 1054 novel miRNAs were found in Control. Among them, nine known miRNAs and 39 novel miRNAs had altered levels in response to HLS treatment. The expression differences of selected miRNAs and their target genes in Control and HLS were verified by qRT-PCR, which showed that novel-m0533-3p was up-regulated and its predicted target malate dehydrogenase encoded gene was clearly down-regulated in HLS at both transcription and the protein level. Moreover, some key carotenoid pathway genes, such asPSY,PDSandLCYBwere all up-regulated in HLS. These findings are providing a new insights into a miRNA-mediated beta-carotene accumulation mechanism inD. salina.