NUCLEOTIDE-SEQUENCES OF INFLUENZA-VIRUS SEGMENTS 1 AND 3 REVEAL MOSAIC STRUCTURE OF A SMALL VIRAL-RNA SEGMENT
NUCLEOTIDE-SEQUENCES OF INFLUENZA-VIRUS SEGMENTS 1 AND 3 REVEAL MOSAIC STRUCTURE OF A SMALL VIRAL-RNA SEGMENT
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DOI：
10.1016/0092-8674(82)90348-8
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发表时间：
1982-01-01
期刊：
影响因子：
64.5
通讯作者：
WINTER, G
中科院分区：
文献类型：
--
作者：
FIELDS, S;WINTER, G
流感病毒的缺陷性干扰RNA是来自病毒片段1、2和3的小片段。提供了人流感病毒株A/PR/8/34的片段1和3的完整核苷酸序列，并且确定了A/NT/60/68的小RNA片段（显然是一种缺陷干扰RNA）的序列来自片段3中的5个独立区域和片段1中的1个区域。这些区域位于2个亲本区段的末端附近，以交替方式排列在小RNA区段中;因此，来源于5“末端附近的区域与来源于3”末端附近的区域相邻。小片段显然是在正链合成过程中产生的，因为病毒聚合酶在模板中富含尿苷的序列处暂停，并在另一个位点重新启动合成。
Defective interfering RNA of influenza virus are small segments derived from viral segments 1, 2 and 3. The complete nucleotide sequences of segments 1 and 3 from the human influenza strain A/PR/8/34 are presented and the sequence of a small RNA segment from A/NT/60/68, apparently a defective interfering RNA, was determined to be derived from 5 separate regions in segment 3 and 1 region in segment 1. These regions, which are located near the termini of the 2 parental segments, are arranged in the small RNA segment in an alternating fashion; thus a region derived from near a 5'' terminus is adjacent to a region derived from near a 3'' terminus. The small segment apparently is generated during positive strand synthesis as a result of the viral polymerase pausing at uridine-rich sequences in the template and reinitiating synthesis at another site.