A molecular approach to leukemogenesis: mouse lymphomas contain an activated c-ras oncogene.
A molecular approach to leukemogenesis: mouse lymphomas contain an activated c-ras oncogene.
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白血病发生的分子方法：小鼠淋巴瘤含有激活的 c-ras 癌基因。
DOI：
10.1073/pnas.81.1.202
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发表时间：
1984
影响因子：
11.1
通讯作者：
Pellicer,A
中科院分区：
文献类型：
--
作者：
Guerrero,I;Calzada,P;Mayer,A;Pellicer,A
通过用致癌物和伽马射线诱发小鼠胸腺瘤，我们研究了肿瘤DNA在啮齿动物成纤维细胞中诱导病灶的可能性。使用的肿瘤中有很高比例转化了培养的细胞，致癌表型与额外的c-ras基因家族拷贝分离。激活的基因似乎具有选择性，因为到目前为止，所有被分析的致癌物诱导的肿瘤都激活了N-ras基因，而辐射诱导的肿瘤则激活了K-ras基因。K-ras基因是柯尔斯滕小鼠肉瘤病毒中病毒ras癌基因的细胞内对应基因，但N-ras基因尚未在逆转录病毒中发现。转化的细胞在RNA和蛋白质水平上癌基因的表达明显增加。该模型系统可能成为白血病发生研究的有力工具。
By inducing mouse thymomas with carcinogens and gamma-radiation, we have studied the potential of tumor DNA to induce foci in rodent fibroblasts. A high percentage of the tumors used transformed the cultured cells, and the oncogenic phenotype segregated with extra copies of the c-ras gene family. There appears to be selectivity in the activated gene because so far all analyzed tumors induced by carcinogen have activated the N-ras gene, and those induced by radiation have activated the K-ras gene. The K-ras gene is the cellular counterpart of the viral ras oncogene in Kirsten murine sarcoma virus, but the N-ras has not yet been found in a retrovirus. The transformed cells have a marked increase in expression of the oncogene at the RNA and protein level. This model system might be a powerful tool in the study of leukemogenesis.