The utility of molecular diagnostics to predict recurrence of head and neck carcinoma.
The utility of molecular diagnostics to predict recurrence of head and neck carcinoma.
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DOI：
10.1038/bjc.2012.213
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发表时间：
2012-09-25
影响因子：
8.8
通讯作者：
Partridge, M.
中科院分区：
文献类型：
--
作者：
Murillo, C. Pena;Huang, X.;Hills, A.;McGurk, M.;Lyons, A.;Jeannon, J-P;Odell, E.;Brown, A.;Lavery, K.;Barrett, W.;Sherriff, M.;Brakenhoff, R.;Partridge, M.
局部复发是口腔鳞状细胞癌术后治疗失败的主要原因。分子诊断有可能改善临床病理学参数，以预测这种复发并计划辅助治疗。最常用的测试是基于检测 TP53 突变，但对于携带野生型基因的病例需要替代方法。使用基于 TP53 和 Ly-6D 标记物检测的精心优化的分子诊断技术对 102 例具有肿瘤邻近边缘（通过显微镜检查被认为是清晰边缘）的病例进行了检查。这些标记也被组合起来以提供双重方法。当比较肿瘤邻近的阳性和清晰的切缘时，双分子诊断发现发生局部区域复发的可能性显着增加的病例（P=0.0001）。当切除边缘的间隙为 5 毫米或更小时，这些测试最有用。与既定的临床病理学参数相比，基于 TP53 的诊断是局部区域复发的更好预测因子。基于 TP53 的优化诊断可快速识别边缘接近的重要病例亚组，这些病例将受益于新的治疗方式以降低复发风险。
Locoregional recurrence is the major cause of treatment failure after surgery for oral squamous cell carcinoma. Molecular diagnostics have the potential to improve on clinicopathological parameters to predict this recurrence and plan adjuvant treatment. The test most frequently applied is based on detecting TP53 mutations, but alternative methodology is required for cases that harbour the wild-type gene. One hundred and two cases with tumour-adjacent margins, considered to be clear margins by microscopy, were examined using carefully optimised molecular diagnostics based on detection of the TP53 and Ly-6D markers. The markers were also combined to provide a dual approach. The dual molecular diagnostic identified cases with a significant increase in the probablility of developing locoregional recurrence when tumour-adjacent positive and clear margins were compared (P=0.0001). These tests were most useful when the clearance at the resection margins was 5 mm or less. The TP53-based diagnostic was a better predictor of locoregional recurrence than established clinicopathological parameters. The optimised TP53-based diagnostic rapidly identifies an important subgroup of cases with close margins that will benefit from new treatment modalities to reduce the risk of recurrence.
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影响因子：
7.3
作者：
van Houten, VMM;Tabor, MP;Brakenhoff, RH
通讯作者：
Brakenhoff, RH
影响因子：
5
作者：
Nieuwenhuis, EJC;Leemans, CR;Brakenhoff, RH
通讯作者：
Brakenhoff, RH
DOI：
10.1158/1078-0432.ccr-09-1433
发表时间：
2009-12-15
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Poeta ML;Manola J;Goldenberg D;Forastiere A;Califano JA;Ridge JA;Goodwin J;Kenady D;Saunders J;Westra W;Sidransky D;Koch WM
通讯作者：
Koch WM
在头颈癌患者的手术边缘中最小残留癌的分子检测。
DOI：
10.3233/clo-2009-0474
发表时间：
2009
期刊：
Cellular oncology : the official journal of the International Society for Cellular Oncology
影响因子：
--
作者：
Graveland AP;de Maaker M;Braakhuis BJ;de Bree R;Eerenstein SE;Bloemena E;Leemans CR;Brakenhoff RH
通讯作者：
Brakenhoff RH
影响因子：
11.5
作者：
Huang, Xiaohong;Pateromichelakis, Stelios;Partridge, Max
通讯作者：
Partridge, Max