Controlled manipulation of molecular samples with the nanoManipulator
Controlled manipulation of molecular samples with the nanoManipulator
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DOI：
10.1109/3516.847092
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发表时间：
2000-06-01
影响因子：
6.4
通讯作者：
Taylor, RM
中科院分区：
文献类型：
--
作者：
Guthold, M;Falvo, MR;Taylor, RM
要想在诸如建造更强大的计算机、测量生物样品的材料特性或探索原子水平上的基本物理定律等各种问题上取得进一步的进展，就需要进入这个世界。nanoManipulator系统为原子力显微镜（AFM）添加了一个虚拟现实界面，从而提供了一种工具，可供科学家以可控的方式对纳米尺寸的分子结构进行成像和操纵。当AFM针尖扫描样品时，针尖-样品相互作用力被监测，其。进而可以产生关于样品的摩擦、机械、材料和拓扑性质的信息。计算机图形用于为用户重建表面，颜色或轮廓覆盖以指示额外的数据集。此外，通过软件连接到尖端的力反馈触笔允许用户直接与大分子互动。该系统正被用于研究碳纳米管（CNT's）、脱氧核糖核酸（DNA）、纤维蛋白、腺病毒和烟草花叶病毒。纳米管已经被弯曲，平移和旋转，以了解它们的机械性能，并在分子水平上研究摩擦。利用原子力显微镜光刻技术结合纳米机械手，研究了碳纳米管的机电特性。DNA和纤维蛋白纤维的断裂力已经被测量，病毒的弹性模量正在研究中。现在还可以将该系统插入扫描电子显微镜中，即使在AFM针尖被用于操作时，扫描电子显微镜也可以为用户提供样品的连续图像。请研究者按照www.example.com网站上的描述申请使用该系统。
Making further advances in such diverse problems as building more powerful computers, measuring material properties of biological samples, or exploring fundamental physical laws on the atomic level requires gaining access to the nanoworld. The nanoManipulator system adds a virtual-reality interface to an atomic-force microscope (AFM), thus providing a tool that can be used by scientists to image and manipulate nanometer-sized molecular structures in a controlled manner. As the AFM tip scans the sample, the tip-sample interaction forces are monitored, which. in turn, can yield information about the frictional, mechanical, material, and topological properties of the sample. Computer graphics are used to reconstruct the surface for the user, with color or contours overlaid to indicate additional data sets. Moreover, a force feedback stylus, which is connected to the tip via software, allows the user to directly interact with the macromolecules. This system is being used to investigate carbon nanotubes (CNT's), deoxyribonucleic acid (DNA), fibrin, adeno-and tobacco mosaic virus. Nanotubes have been bent, translated, and rotated to understand their mechanical properties and to investigate friction on the molecular level. Using AFM lithography in combination with the nanoManipulator, the electromechanical properties of CNT's ale being investigated. The rupture forces of DNA and fibrin fibers have been measured and the elastic moduli of viruses are being studied. It is now also possible to insert this system into a scanning electron microscope which pro,ides the user with continuous images of the sample, even while the AFM tip is being used for manipulations. Investigators are invited to apply to use the system as described on the web at http://www.cs.unc.edu/Research/nano/doc/biovisit.html.