Efflux pump inhibitors reduce the invasiveness of Pseudomonas aeruginosa
Efflux pump inhibitors reduce the invasiveness of Pseudomonas aeruginosa
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DOI：
10.1016/j.ijantimicag.2009.06.007
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发表时间：
2009-10-01
影响因子：
10.8
通讯作者：
Kaku, Mitsuo
中科院分区：
文献类型：
--
作者：
Hirakata, Yoichi;Kondo, Akira;Kaku, Mitsuo
外排系统被认为是铜绿假单胞菌产生抗菌素耐药性的原因。铜绿假单胞菌PAO1的mexAB-oprM缺失株侵袭Madin-Darby犬肾(MDCK)细胞的能力受到损害，这表明铜绿假单胞菌利用MexAB-oprM系统输出侵袭决定因素。研究了外排泵抑制剂(EPI)对野生型(WT)铜绿假单胞菌PAO1及其MexAB-OprM高产NALB菌株侵袭能力的影响。EPIS对PAO1、WT和NALB的侵袭力均有抑制作用，且呈浓度依赖性。与PAβN相比，D13-9001的侵袭力下降幅度更大，EPIS被认为有助于降低铜绿假单胞菌的侵袭力和耐药性，因此有望成为一种新的抗感染药物。(C)2009年爱思唯尔公司和国际化疗学会。版权所有。
Efflux systems are thought to contribute to antimicrobial resistance in Pseudomonas aeruginosa. The mexAB-oprM deletion strain of P. aeruginosa PAO1 is compromised in its capacity to invade Madin-Darby canine kidney (MDCK) cells, suggesting that P. aeruginosa exports invasion determinants using a MexAB-OprM system. The influences of efflux pump inhibitors (EPIs), including the broad-spectrum EPI Phe-Arg-beta-naphthylamide (PA beta N) and MexAB-OprM-specific EPI D13-9001, on the invasion of wild-type (WT) P. aeruginosa PAO1 and its MexAB-OprM-overproducing nalB strain were examined. The invasiveness of PAO1 WT and nalB strains was inhibited in the presence of EPIs in a concentration-dependent manner. Reduction of the invasiveness of both strains was greater for D13-9001 compared with PA beta N. EPIs are thought to be useful in reducing the invasiveness and antimicrobial resistance of P. aeruginosa and thus may be promising as new anti-infectious agents. (C) 2009 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.