3D Printed Molds Encompassing Carbon Composite Electrodes To Conduct Multisite Monitoring in the Entire Colon
3D Printed Molds Encompassing Carbon Composite Electrodes To Conduct Multisite Monitoring in the Entire Colon
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DOI：
10.1021/acs.analchem.7b03148
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发表时间：
2017-11-07
影响因子：
7.4
通讯作者：
Patel, Bhavik Anil
中科院分区：
文献类型：
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作者：
Patel, Nirav;Fagan-Murphy, Aidan;Patel, Bhavik Anil
结肠的活动受化学信号的调节，其中5-羟色胺(5-羟色胺)是关键的递质。到目前为止，使用一小段结肠组织上的微电极已经实现了对粘膜5-羟色胺溢出的监测；然而，对于这种测量是否反映了整个结肠的5-羟色胺信号，人们知之甚少。这项研究的重点是开发一种可用于对整个结肠的5-羟色胺溢出进行多点测量的电化学阵列设备。制造了一种3D打印模具，可以容纳6个多壁碳纳米管复合电极，并在电极和组织之间提供沿整个结肠长度的固定距离。在使用6个月和24个月大的小鼠的结肠进行体外测量之前，对电极的灵敏度、稳定性和串扰进行了评估。由于复合电极可能具有高度的可变性，因此对于给定阵列，电极之间需要归一化系数。该装置具有从肠道组织中测量5-羟色胺所需的灵敏度和稳定性。随着年龄的增长，5-羟色胺溢出的区域特异性变化，其中，由于5-羟色胺转运体(SERT)的损伤/丢失，远端结肠的5-羟色胺溢出增加。我们的策略可以用于开发不同大小和几何形状的阵列，这可以为大规模组织测量提供实用的解决方案。
The activity of the colon is regulated by chemical signaling; of which serotonin (5-HT) is a key transmitter. Monitoring of mucosal 5-HT overflow has been achieved to date using microelectrodes on a small segment of colonic tissue; however, little is known if such measurements are reflective with regards to 5-HT signaling from the entire colon. This study focused on developing an electrochemical array device that could be utilized to conduct multisite measurements of 5-HT overflow from the entire colon. A 3D printed mold was fabricated that could house 6 multiwall carbon nanotube composite electrodes and provide a fixed distance between the electrodes and the tissue along the entire length of the colon. The electrodes were assessed for sensitivity, stability, and crosstalk before conducting in vitro measurements using colons obtained from 6- and 24-month old mice. As composite electrodes can have a high degree of variability, normalization factors were required between electrodes for a given array. The device had the sensitivity and stability required for 5-HT measurements from intestinal tissue. Regio-specific changes in 5-HT overflow were observed with age, where increases in 5-HT overflow were observed in the distal colon due to an impairment/loss in the serotonin transporter (SERT). Our strategy can be utilized to develop arrays of varying sizes and geometries, which can offer practical solutions for large-scale tissue measurements.