DERIVATIONS OF RELATIVE BIOLOGICAL EFFECTIVENESS FOR THE HIGH-LET RADIATIONS PRODUCED DURING BORON NEUTRON-CAPTURE IRRADIATIONS OF THE 9L RAT GLIOSARCOMA IN-VITRO AND IN-VIVO
DERIVATIONS OF RELATIVE BIOLOGICAL EFFECTIVENESS FOR THE HIGH-LET RADIATIONS PRODUCED DURING BORON NEUTRON-CAPTURE IRRADIATIONS OF THE 9L RAT GLIOSARCOMA IN-VITRO AND IN-VIVO
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DOI：
10.1016/0360-3016(93)90533-2
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发表时间：
1993-12-01
影响因子：
7
通讯作者：
AMOLS, HI
中科院分区：
文献类型：
--
作者：
CODERRE, JA;MAKAR, MS;AMOLS, HI
目的：硼中子俘获疗法过程中产生的高线性能量传递粒子的相对生物学效应（RBE）值通常基于理论考量或体外实验。本研究的目的是独立确定硼中子俘获疗法所有剂量成分的RBE值。
方法和材料：获取了9L大鼠胶质肉瘤细胞在布鲁克海文医学研究反应堆热中子束中体外照射以及作为脑内肿瘤照射后的克隆形成细胞存活数据。使用细胞存活的线性二次模型对这些数据进行分析，以得出所有射束成分以及多种不同硼化合物的实测RBE值。
结果：在无硼的情况下，氮俘获产生的质子（N - 14（n，p）C - 14）以及快中子散射（H - 1（n，n'）p）反应的综合效应在体外产生的RBE为3.7，在体内/体外切除实验中为3.2（与以1%细胞存活率为终点的250 kVp X射线相比）。在存在氨基酸对 - 硼苯丙氨酸、巯基十二硼酸盐单体或二聚体或硼酸的情况下，根据反应堆照射后的细胞存活数据计算出B - 10（n，α）Li - 7反应产物的表观RBE。B - 10（n，α）Li - 7反应的表观RBE值在1.2到9.8之间，取决于所使用的硼化合物。体外研究的RBE值始终高于体内/体外研究的RBE值。在任何条件下，对 - 硼苯丙氨酸的B - 10（n，α）Li - 7反应的表观RBE值都高于所测试的任何其他硼化合物。
结论：普遍接受的总剂量中快中子和N - 14（n，p）C - 14反应成分的RBE值过低。为B - 10（n，α）Li - 7反应计算的表观RBE值依赖于化合物，并且与B - 10相对于神经胶质瘤细胞核的分布差异相符。
Purpose: Relative biological effectiveness (RBE) values for the high linear-energy-transfer particles produced during boron neutron capture therapy have generally been based on theoretical considerations or on in vitro experiments. The purpose of this study was to independently determine RBE values for all of the boron neutron capture therapy dose components.Methods and Materials: Clonogenic cell survival data were obtained for 9L rat gliosarcoma cells irradiated in the Brookhaven Medical Research Reactor thermal neutron beam both in vitro and as an intracerebral tumor. These data were analyzed using the linear quadratic model for cell survival to derive measured RBE values for all beam components and for a number of different boron compounds.Results: In the absence of boron, the combined effects of the protons from the nitrogen capture, N-14(n,p)C-14, and the fast neutron scatter, H-1(n,n')p, reactions generated RBEs of 3.7 in vitro and 3.2 in an in vivo/in vitro excision assay, compared to 250 kVp X rays using an end point of 1% cell survival. Apparent RBEs for the B-10(n,alpha)Li-7 reaction products were calculated from cell survival data following reactor irradiations in the presence of the amino acid p-boronophenylalanine, the sulfhydryl dodecaborate monomer or dimer, or boric acid. Apparent RBEs for the B-10(n,alpha)Li-7 reaction ranged from 1.2 to 9.8 depending on which boron compound was used. RBEs from the in vitro studies were consistently higher than from the in vivo/in vitro studies. Under any conditions, the apparent RBE for the B-10(n,alpha)Li-7 reaction with p-boronophenylalanine was higher than that with any other boron compound tested.Conclusions: Generally accepted RBE values for the fast neutron and N-14(n,p)C-14 reaction components of the total dose are too low. The apparent RBEs calculated for the B-10(n,alpha)Li-7 reaction were compound-dependent and consistent with differences in the distribution of B-10 relative to glioma cell nuclei.