Photosynthesis Is Widely Distributed among Proteobacteria as Demonstrated by the Phylogeny of PufLM Reaction Center Proteins.
Photosynthesis Is Widely Distributed among Proteobacteria as Demonstrated by the Phylogeny of PufLM Reaction Center Proteins.
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DOI：
10.3389/fmicb.2017.02679
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发表时间：
2017
影响因子：
5.2
通讯作者：
Neulinger SC
中科院分区：
文献类型：
--
作者：
Imhoff JF;Rahn T;Künzel S;Neulinger SC
在不同的细菌门中采用两种不同的光系统进行细菌叶绿素介导的光合能量转换。那些利用光系统II型光合机构的细菌包括光合紫色细菌（变形菌门）、芽单胞菌和绿挠曲菌及其光合亲缘菌。光合反应中心的蛋白质PufL和PufM是必需的组分，并且是所有具有II型光合装置的细菌所共有的，包括厌氧以及有氧光养变形菌。因此，PufL和PufM蛋白及其基因是评估光合机构的发育和研究自然界中利用这种光系统的细菌多样性的理想工具。几乎完整的pufLM基因序列和衍生的蛋白质序列从152型菌株和45个额外的菌株的光养变形菌采用光系统II进行了比较。结果给出了有趣的和全面的见解的光合机构和明确定义Chromatiales，Rhododocyanales，Sphingomonadales作为主要群体不同于其他Alphaproteobacteria，Betaproteobacteria和柄杆菌目（Brevundimonas subvibrioides）。在使用细菌叶绿素B代替细菌叶绿素a的那些细菌的PufLM序列之间存在特殊关系。一个明确的系统发育协会的好氧光合紫色细菌厌氧紫色细菌根据其PufLM序列表明多个进化线从厌氧到好氧光合紫色细菌。讨论了pufLM基因序列对光合细菌环境多样性研究的影响，指出了在环境样品中进行光合细菌种水平鉴定的可能性。
Two different photosystems for performing bacteriochlorophyll-mediated photosynthetic energy conversion are employed in different bacterial phyla. Those bacteria employing a photosystem II type of photosynthetic apparatus include the phototrophic purple bacteria (Proteobacteria), Gemmatimonas and Chloroflexus with their photosynthetic relatives. The proteins of the photosynthetic reaction center PufL and PufM are essential components and are common to all bacteria with a type-II photosynthetic apparatus, including the anaerobic as well as the aerobic phototrophic Proteobacteria. Therefore, PufL and PufM proteins and their genes are perfect tools to evaluate the phylogeny of the photosynthetic apparatus and to study the diversity of the bacteria employing this photosystem in nature. Almost complete pufLM gene sequences and the derived protein sequences from 152 type strains and 45 additional strains of phototrophic Proteobacteria employing photosystem II were compared. The results give interesting and comprehensive insights into the phylogeny of the photosynthetic apparatus and clearly define Chromatiales, Rhodobacterales, Sphingomonadales as major groups distinct from other Alphaproteobacteria, from Betaproteobacteria and from Caulobacterales (Brevundimonas subvibrioides). A special relationship exists between the PufLM sequences of those bacteria employing bacteriochlorophyll b instead of bacteriochlorophyll a. A clear phylogenetic association of aerobic phototrophic purple bacteria to anaerobic purple bacteria according to their PufLM sequences is demonstrated indicating multiple evolutionary lines from anaerobic to aerobic phototrophic purple bacteria. The impact of pufLM gene sequences for studies on the environmental diversity of phototrophic bacteria is discussed and the possibility of their identification on the species level in environmental samples is pointed out.