Boundary Bypass Activity in the Abdominal-B Region of the Drosophila Bithorax Complex is Position Dependent and Regulated.
Boundary Bypass Activity in the Abdominal-B Region of the Drosophila Bithorax Complex is Position Dependent and Regulated.
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果蝇双胸复合体腹部 B 区的边界旁路活动是位置依赖性和调节性的。
DOI：
10.1101/2023.06.06.543971
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Schedl,Paul
中科院分区：
文献类型：
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作者：
Kyrchanova,Olga;Ibragimov,Airat;Postika,Nikolay;Georgiev,Pavel;Schedl,Paul
Abd-B在A5-A8腹段的表达受IAB-5-IAB-8四个调控结构域的调控。每个域都有一个启动器元素(设置活动状态)、维持该状态的元素和特定于组织的增强剂。为了确保它们的功能自主性，每个域都用边界元素(MCP、FAB-7、FAB-7和FAB-8)括起来。除了阻止相邻调控结构域之间的串扰外，结构域还必须具有旁路活性，以便相关的调控结构域可以“跳过”中间的边界并激活Abd-B启动子。在这里报道的研究中，我们调查了控制旁路活动的参数。我们发现，Fab-7和Fab-8边界中的旁路元件必须位于负责驱动Abd-B表达的调控结构域中。我们建议，旁路活动也可能受到监管。
Expression ofAbdominal-B(Abd-B) in abdominal segments A5–A8 is controlled by four regulatory domains,iab-5–iab-8. Each domain has an initiator element (which sets the activity state), elements that maintain this state and tissue-specific enhancers. To ensure their functional autonomy, each domain is bracketed by boundary elements (Mcp,Fab-7,Fab-7andFab-8). In addition to blocking crosstalk between adjacent regulatory domains, theFabboundaries must also have bypass activity so the relevant regulatory domains can ‘jump over’ intervening boundaries and activate theAbd-Bpromoter. In the studies reported here we have investigated the parameters governing bypass activity. We find that the bypass elements in theFab-7andFab-8boundaries must be located in the regulatory domain that is responsible for drivingAbd-Bexpression. We suggest that bypass activity may also be subject to regulation.