Mutations in PEP4 locus of Saccharomyces cerevisiae block final step in maturation of two vacuolar hydrolases.
Mutations in PEP4 locus of Saccharomyces cerevisiae block final step in maturation of two vacuolar hydrolases.
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DOI：
10.1073/pnas.80.2.510
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发表时间：
1983
影响因子：
11.1
通讯作者：
G. Zubenko;F. J. Park;E. W. Jones
中科院分区：
文献类型：
--
作者：
G. Zubenko;F. J. Park;E. W. Jones
酵母细胞中羧肽酶 Y (EC 3.4.16.1) 和蛋白酶 A (EC 3.4.23.6) 的生物合成涉及一系列翻译后事件，最后一个事件取决于 PEP4 基因提供的功能。由于 pep4 突变导致至少三种另外的液泡水解酶的活性水平降低 90-95%，因此这些酵母液泡水解酶（也许是所有酵母液泡水解酶）很可能是作为无活性前体合成的，它们通过依赖于 PEP4 基因提供的功能的常见机制成熟。 pep4 突变显示出对蛋白酶 A 和 B 的活性水平有明显的基因剂量效应，但对羧肽酶 Y 的活性水平没有影响。这种效应的出现似乎是因为成熟机制能够区分这些水解酶前体。
The biosynthesis of carboxypeptidase Y (EC 3.4.16.1) and proteinase A (EC 3.4.23.6) in yeast cells involves a series of posttranslational events, the last of which is dependent upon a function supplied by the PEP4 gene. Because pep4 mutations result in a 90-95% reduction in the levels of activity of at least three additional vacuolar hydrolases, it is likely that these, and perhaps all, yeast vacuolar hydrolases are synthesized as inactive precursors, which mature by a common mechanism that depends upon a function supplied by the PEP4 gene. The pep4 mutation shows an apparent gene dosage effect on levels of activity of proteinases A and B but not on the level of activity of carboxypeptidase Y. This effect appears to come about because the maturation machinery is capable of discriminating among these hydrolase precursors.