Human proximal tubular cell responses to angiotensin II analyzed using DNA microarray.
Human proximal tubular cell responses to angiotensin II analyzed using DNA microarray.
复制标题
使用 DNA 微阵列分析人近端肾小管细胞对血管紧张素 II 的反应。
DOI：
10.1016/s0014-2999(03)01382-7
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发表时间：
2003
影响因子：
5
通讯作者：
Hammond,Timothy
中科院分区：
文献类型：
--
作者：
Braam,Branko;Allen,Pat;Benes,Ed;Koomans,HeinA;Navar,LGabriel;Hammond,Timothy
Angiotensin II has been shown to exert complex effects on proximal tubular cell function and growth. To assess some of the direct effects on proximal tubular cells, changes in gene expression of selected cellular pathways were determined after exposure to angiotensin II. We used DNA microarrays to analyze multiple gene expression responses to increasing angiotensin II concentrations. Human proximal tubular cells were grown in flasks, and the presence of angiotensin type 1 receptor was confirmed by Western blot analysis. At passages 4–6, these cells were exposed to angiotensin II and harvested 4 h later and mRNA of the cells was extracted; 2 μg of mRNA was fluorescently conjugated for cDNA microarray hybridization. A custom-made DNA microarray was designed by selecting 300 human genes from 10 different functional systems and amplifying clones using polymerase chain reaction. Cells were subjected to 10 and 100 nM angiotensin II with paired untreated cells as controls. RNA was isolated, reverse transcribed, labeled and hybridized to the arrays and the ratios calculated. Ratios of ≥2.0 and ≤0.5 were considered significant. Coordinated changes were observed in genes of the hepatocyte nuclear factor 3 family (NHF3; HNF3A, HNF3B and HNF3G), in the E2F genes (E2F1, E2F3) and the interferon regulatory factors IRF1 and IRF5. Induction of the expression of transcription factors points towards complex regulation of gene expression upon angiotensin II exposure. Three genes involved in the dampening of oxidative stress were enhanced. Taken together, brief exposure of human tubular epithelial cells to angiotensin II elicited a marked induction of nuclear factors, antioxidant genes and hormones and hormone receptor genes. The quick activation of transcription factors by angiotensin II indicates that angiotensin II can directly initiate a cascade of expressional events in proximal tubular cells.
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血管紧张素 II 激活人单核细胞中的促炎转录因子核因子 -kappaB。
DOI：
--
发表时间：
1999
期刊：
Biochemical and Biophysical Research Communications - BBRC
影响因子：
--
作者：
R. Kranzhöfer;M. Browatzki;Joachim W. Schmidt;Wolfgang Kübler
通讯作者：
Wolfgang Kübler
DOI：
--
发表时间：
1999-04
期刊：
Journal of the American Society of Nephrology : JASN
影响因子：
--
作者：
L. Navar;L. Harrison-Bernard;J. Imig;C.-T. Wang;L. C̆ervenka;Kenneth D. Mitchell
通讯作者：
L. Navar;L. Harrison-Bernard;J. Imig;C.-T. Wang;L. C̆ervenka;Kenneth D. Mitchell
影响因子：
--
作者：
BRAAM, B;MITCHELL, KD;NAVAR, LG
通讯作者：
NAVAR, LG
影响因子：
13.6
作者：
Nasr, Khaled;Viswanathan, Pooja;Nieder, Andreas
通讯作者：
Nieder, Andreas
影响因子：
20.1
作者：
Fukui, T;Ishizaka, N;Griendling, KK
通讯作者：
Griendling, KK