Sterols and Sphingolipids as New Players in Cell Wall Building and Apical Growth of Nicotiana tabacum L. Pollen Tubes.
Sterols and Sphingolipids as New Players in Cell Wall Building and Apical Growth of Nicotiana tabacum L. Pollen Tubes.
复制标题
DOI：
10.3390/plants12010008
复制
发表时间：
2022-12-20
期刊：
影响因子：
--
通讯作者：
Moscatelli A
中科院分区：
文献类型：
--
作者：
Stroppa N;Onelli E;Moreau P;Maneta-Peyret L;Berno V;Cammarota E;Ambrosini R;Caccianiga M;Scali M;Moscatelli A
花粉管是顶端生长的细胞，它创造了安全的路线，将精子细胞运送到胚囊进行双受精。最近的研究纯化和生化特性的洗涤剂不溶性膜从烟草花粉管。这些微结构域被称为脂筏，富含甾醇和鞘脂，并参与进化上遥远的生物体（如真菌和哺乳动物）的细胞极化。肌动蛋白在烟草花粉管洗涤剂不溶性膜的存在下，这些领域的顶端质膜上的优先分布，鼓励我们制定有趣的假设，甾醇和鞘脂可能是一个“特质D '工会”之间的肌动蛋白动力学和极化分泌的尖端。要解开烟草花粉管生长中的甾醇和鞘脂的作用，我们使用鲨烯蛋白和myriocin，抑制剂甾醇和鞘脂的生物合成，分别，以确定脂质修饰是否影响肌动蛋白边缘形态和动力学，导致清晰的区域组织和细胞壁沉积的变化，从而表明这些脂质在成功受精中发挥的作用。
Pollen tubes are tip-growing cells that create safe routes to convey sperm cells to the embryo sac for double fertilization. Recent studies have purified and biochemically characterized detergent-insoluble membranes from tobacco pollen tubes. These microdomains, called lipid rafts, are rich in sterols and sphingolipids and are involved in cell polarization in organisms evolutionarily distant, such as fungi and mammals. The presence of actin in tobacco pollen tube detergent-insoluble membranes and the preferential distribution of these domains on the apical plasma membrane encouraged us to formulate the intriguing hypothesis that sterols and sphingolipids could be a “trait d’union” between actin dynamics and polarized secretion at the tip. To unravel the role of sterols and sphingolipids in tobacco pollen tube growth, we used squalestatin and myriocin, inhibitors of sterol and sphingolipid biosynthesis, respectively, to determine whether lipid modifications affect actin fringe morphology and dynamics, leading to changes in clear zone organization and cell wall deposition, thus suggesting a role played by these lipids in successful fertilization.
登录
查看更多内容
影响因子：
7.4
作者：
Baluska, F;Hlavacka, A;Volkmann, D
通讯作者：
Volkmann, D
影响因子：
4.1
作者：
Bessueille, Laurence;Sindt, Nicolas;Bulone, Vincent
通讯作者：
Bulone, Vincent
影响因子：
4.9
作者：
Alexandersson, E;Saalbach, G;Kjellbom, P
通讯作者：
Kjellbom, P
影响因子：
--
作者：
Aouar, Leila;Chebli, Youssef;Geitmann, Anja
通讯作者：
Geitmann, Anja
影响因子：
7.4
作者：
Chebli, Youssef;Kaneda, Minako;Geitmann, Anja
通讯作者：
Geitmann, Anja