Effects of a probiotic, Enterococcus faecium, on growth performance, intestinal morphology, immune response, and cecal microflora in broiler chickens challenged with Escherichia coli K88
Effects of a probiotic, Enterococcus faecium, on growth performance, intestinal morphology, immune response, and cecal microflora in broiler chickens challenged with Escherichia coli K88
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DOI：
10.3382/ps.2013-03366
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发表时间：
2013-11-01
期刊：
影响因子：
4.4
通讯作者：
Yang, C. M.
中科院分区：
文献类型：
--
作者：
Cao, G. T.;Zeng, X. F.;Yang, C. M.
研究了饲喂脱水屎肠球菌对大肠杆菌K88感染肉鸡生产性能、免疫应答和盲肠菌群的影响。将288只1日龄鸡随机分为4组：阴性对照鸡（N-con）饲喂基础日粮，不攻毒。coli K88;阳性对照鸡（P-con）饲喂基础饲料并用E. coli K88;屎肠球菌（Ef）以1 × 10（9）cfu/kg饲料感染，并用E.大肠杆菌K88;以及饲喂包括10 mg/kg饲料的抗生素硫酸粘杆菌素（Anti）的基础饮食并用E. coli K88。鸟类喂养E.与其他处理组相比，粪肠组在第14、21和28天的体重显著增加（P <0.05），在第21和28天的空肠绒毛高度显著增加（P <0.05）。空肠隐窝深度在饲喂E.粪便或抗生素与P-con处理的鸡相比，在第10、21和28天。鸟类喂养E.在第10、14和21天，粪肠的空肠粘膜中的IL-4浓度高于N-con治疗组（P < 0.05）。感染的鸟类，有或没有E。在第10天和第14天，粪便喂养组的空肠粘膜中肿瘤坏死因子-α和分泌的伊加高于N-con治疗组（P < 0.05）。鸟类喂养E.屎肠中E.大肠杆菌在第14和28天显著高于大肠杆菌对照组（P < 0.05），产气荚膜梭菌在第28天显著低于大肠杆菌对照组（P <0.05），乳酸杆菌在第14和21天显著高于大肠杆菌对照组（P < 0.05），双歧杆菌在第21天显著高于大肠杆菌对照组（P < 0.05）。这些结果表明，E.粪菌能促进肉鸡的生长性能，改善肠道形态，并对盲肠菌群有一定的调控作用。coli K88。
The effects of feeding dehydrated Enterococcus faecium on growth performance, immune response, and cecal microflora in broiler chickens challenged with Escherichia coli K88 were investigated. Two hundred eighty-eight 1-d-old birds were randomly assigned to 4 treatments: negative control birds (N-con) fed a basal diet and not challenged with E. coli K88; positive control birds (P-con) fed a basal diet and challenged with E. coli K88; birds fed a basal diet including dehydrated E. faecium (Ef) at 1 x 10(9) cfu/kg of feed and challenged with E. coli K88; and birds fed a basal diet including the antibiotic colistine sulfate (Anti) at 10 mg/kg of feed and challenged with E. coli K88. Birds fed E. faecium had greater (P < 0.05) BW on d 14, 21, and 28 and greater (P < 0.05) jejunal villus height on d 21 and 28 compared with birds on the other treatments. Jejunal crypt depth was decreased (P < 0.05) in birds fed either E. faecium or antibiotic compared with P-con treatment birds on d 10, 21, and 28. Birds fed E. faecium had a greater (P < 0.05) concentration of IL-4 in their jejunal mucosa than did those in the N-con treatment group on d 10, 14, and 21. Infected birds, with or without E. faecium feeding, had a higher (P < 0.05) tumor necrosis factor-alpha and secreted IgA in their jejunal mucosa than did those in the N-con treatment group on d 10 and 14. Birds fed E. faecium had lower (P < 0.05) concentrations of E. coli on d 14 and 28, less (P < 0.05) Clostridium perfringens on d 28, greater Lactobacillus counts on d 14 and 21, and greater (P < 0.05) Bifidobacterium in their cecal contents on d 21 than did the P-con birds. These results suggest that E. faecium can promote growth performance, improve intestinal morphology, and beneficially manipulate the cecal microflora in broilers challenged with E. coli K88.