Use of a common promoter by two juxtaposed and intronless mouse early embryonic genes, Rnf33 and Rnf35:: Implications in zygotic gene expression
Use of a common promoter by two juxtaposed and intronless mouse early embryonic genes, Rnf33 and Rnf35:: Implications in zygotic gene expression
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DOI：
10.1006/geno.2002.6808
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发表时间：
2002-08-01
期刊：
影响因子：
4.4
通讯作者：
Choo, KB
中科院分区：
文献类型：
--
作者：
Chen, HH;Liu, TYC;Choo, KB
Rnf33和Rnf35是小鼠无名指蛋白基因，在植入前小鼠胚胎中暂时转录，主要在双细胞胚胎阶段。这些基因并置在一个20 kb的基因组区域中，除了5‘非翻译区（5’-UTR）有一个内含子外，它们都是无内含子的。基于对Rnf33/35基因组序列和通过硅矿提取的cDNA序列的分析，我们发现Rnf33和Rnf35 mrna显然是从相同的假定启动子转录而来，并且可能是通过差异的3'切割和聚腺苷酸化产生的相同的前mrna的选择性剪接的产物。我们还在使用非常规5'剪接连接的第二次剪接事件产生的双细胞胚胎中检测到Rnf35的第二种变体。我们对Rnf33和Rnf35转录模式的观察与合子基因转录是混杂的假设一致，并且单独的5'-UTR内含子可能有助于促进高效翻译。
Rnf33 and Rnf35 are mouse RING finger protein genes that are transcribed temporally in the preimplantation mouse embryo, predominantly at the two-cell embryonic stage. The genes are juxtaposed in a 20-kb genomic region and are both intronless except for a single intron in the 5' untranslated region (5'-UTR). Based on analysis of the Rnf33/35 genomic sequence and cDNA sequences derived by in silico mining, we found that the Rnf33 and Rnf35 mRNAs are apparently transcribed from the same putative promoter and may be products of alternative splicing of the same pre-mRNA generated through differential 3' cleavage and polyadenylation. We also detected a second variant of Rnf35 in two-cell embryo generated through a second splicing event using an unconventional 5' splice junction. Our observations on the mode of transcription of Rnf33 and Rnf35 are consistent with the hypothesis that transcription of zygotic genes is promiscuous, and that the solo 5'-UTR intron may serve to facilitate efficient translation.