Upregulation of Glycoprotein Nonmetastatic B by Colony-Stimulating Factor-1 and Epithelial Cell Adhesion Molecule in Hepatocellular Carcinoma Cells
Upregulation of Glycoprotein Nonmetastatic B by Colony-Stimulating Factor-1 and Epithelial Cell Adhesion Molecule in Hepatocellular Carcinoma Cells
复制标题
集落刺激因子 1 和上皮细胞粘附分子对肝细胞癌细胞中糖蛋白非转移性 B 的上调
DOI：
10.3727/096504013x13657689382851
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发表时间：
2012-01-01
影响因子：
3.1
通讯作者：
Chang, Hulin
中科院分区：
文献类型：
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作者：
Tian, Feng;Liu, Chang;Chang, Hulin
近年来，人们一直致力于研究肝细胞癌（HCC）的生物标志物及其作用机制和功能意义。糖蛋白非转移性B（GPNMB）的过度表达发生在皮肤黑色素瘤和乳腺癌中，是一种有吸引力的癌症治疗候选者。然而，对GPNMB在HCC中的表达和调控知之甚少。本研究采用组织化学方法检测GPNMB在肝癌细胞中的表达，并探讨上皮细胞粘附分子（EpCAM）和集落刺激因子（CSF-1）对GPNMB表达的调节作用。我们的研究结果表明，GPNMB水平显着增强，在肝癌与相邻的正常肝组织相比。在肝癌细胞中，GPNMB的表达受EpCAM和CSF-1的调节，部分通过它们共同的下游产物c-myc。总之，这些结果表明，GPNMB的表达在HCC细胞中受到各种蛋白质的高度协调功能的调节，可能是HCC治疗的潜在靶点。
Considerable effort has been made in elucidating the appropriate biomarkers and the mechanism and functional significance of these biomarkers in hepatocellular carcinoma (HCC). Glycoprotein nonmetastatic B (GPNMB) overexpression occurs in cutaneous melanomas and breast cancer, and it is an attractive candidate for cancer therapy. However, little is known about the expression and regulation of GPNMB in HCC. In this study, we investigated the expression of GPNMB in HCC histochemically and tested the regulation effects of the epithelial cell adhesion molecule (EpCAM) and colony-stimulating factor (CSF-1) on the expression of GPNMB in HCC cells. Our results demonstrated that GPNMB levels were significantly enhanced in HCC compared with adjacent normal liver tissues. In HCC cells, GPNMB expression was regulated by EpCAM and CSF-1 partly through their common downstream product c-myc. Taken together, these results suggest that GPNMB, the expression of which was regulated in HCC cells by the highly coordinated function of various proteins, may be a potential target for HCC therapy.