The root knot nematode resistance gene Mi from tomato is a member of the leucine zipper, nucleotide binding, leucine-rich repeat family of plant genes
The root knot nematode resistance gene Mi from tomato is a member of the leucine zipper, nucleotide binding, leucine-rich repeat family of plant genes
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DOI：
10.1105/tpc.10.8.1307
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发表时间：
1998-08-01
期刊：
影响因子：
11.6
通讯作者：
Williamson, VM
中科院分区：
文献类型：
--
作者：
Milligan, SB;Bodeau, J;Williamson, VM
番茄的Mi基因座对根结线虫具有抗性。从番茄基因组DNA中分离得到细菌人工染色体克隆，并对其52 kb的相邻DNA进行了测序。鉴定出3个与已克隆的植物抗病基因相似的开放阅读框。其中两个，Mi-1.1和Mi-1.2，似乎是完整的基因；第三个是假基因。与这些基因杂交的4kb的mRNA存在于番茄的根中。利用克隆的Mi-1.1和Mi-1.2的互补研究表明，Mi-1.2，而不是Mi-1.1，足以赋予感病番茄品系以抗性分离的转化子后代。克隆到的与Mi-1.2最相似的基因是prf，这是一个番茄抗丁香假单胞菌所需的基因。PrF和Mi-1.2有几个共同的结构基序，包括一个核苷酸结合位点和一个富含亮氨酸的重复区域，这是一个植物蛋白家族的特征，包括几个对病毒、细菌、真菌和现在的线虫的抗性所必需的蛋白。
The Mi locus of tomato confers resistance to root knot nematodes. Tomato DNA spanning the locus was isolated as bacterial artificial chromosome clones, and 52 kb of contiguous DNA was sequenced. Three open reading frames were identified with similarity to cloned plant disease resistance genes. Two of them, Mi-1.1 and Mi-1.2, appear to be intact genes; the third is a pseudogene. A 4-kb mRNA hybridizing with these genes is present in tomato roots. Complementation studies using cloned copies of Mi-1.1 and Mi-1.2 indicated that Mi-1.2, but not Mi-1.1, is sufficient to confer resistance to a susceptible tomato line with the progeny of transformants segregating for resistance. The cloned gene most similar to Mi-1.2 is Prf, a tomato gene required for resistance to Pseudomonas syringae. Prf and Mi-1.2 share several structural motifs, including a nucleotide binding site and a leucine-rich repeat region, that are characteristic of a family of plant proteins, including several that are required for resistance against viruses, bacteria, fungi, and now, nematodes.