REGULATION OF INTERMOLECULAR AND INTRAMOLECULAR LIGATION WITH T4 DNA-LIGASE IN THE PRESENCE OF POLYETHYLENE-GLYCOL
REGULATION OF INTERMOLECULAR AND INTRAMOLECULAR LIGATION WITH T4 DNA-LIGASE IN THE PRESENCE OF POLYETHYLENE-GLYCOL
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DOI：
10.1093/nar/14.19.7617
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发表时间：
1986-10-10
影响因子：
14.9
通讯作者：
OBAYASHI, A
中科院分区：
文献类型：
--
作者：
HAYASHI, K;NAKAZAWA, M;OBAYASHI, A
聚乙二醇（PEG）刺激与T4 DNA连接酶的连接。在10% (w/v) PEG 6000溶液中，单价阳离子只增强了分子间的连接，而在没有它们的情况下，分子间和分子内的连接都发生了。PEG 6000溶液中的二价阳离子或多胺也会引起类似的刺激。这种连接酶的性质可用于控制分子间和分子内连接的程度。在10% PEG 6000溶液中，阳离子或多胺连接对分子间连接有效。在6.0% ~ 10%的PEG 6000溶液中，不含阳离子或多胺的结扎对分子内结扎有效。
Polyethlene glycol (PEG) stimulates ligation with T4 DNA ligase. In 10% (w/v) PEG 6000 solutions, only intermolecular ligation is enhanced by monovalent cations, while both inter- and intramolecular ligation occur without their presence. Similar stimulation was also caused by divalent cations or polyamines in the PEG 6000 solutions. Such properties of the ligase could be applied to control the extent of inter- and intramolecular ligation. Ligation with cations or polyamines in 10% PEG 6000 solutions was effective for intermolecular ligation. Ligation without cations or polyamines in 6.0% to 10% PEG 6000 solutions was effective for intramolecular ligation.