Carbon nanotube delivery of the GFP gene into mammalian cells
Carbon nanotube delivery of the GFP gene into mammalian cells
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碳纳米管将 GFP 基因传递到哺乳动物细胞中
DOI：
10.1002/cbic.200500227
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发表时间：
2006-02-01
期刊：
影响因子：
3.2
通讯作者：
Yan, XY
中科院分区：
文献类型：
--
作者：
Gao, LZ;Nie, L;Yan, XY
外源基因在哺乳动物细胞中的表达和操纵已成为生物医学研究的支柱。因此，改进有效地将基因转移到多种细胞类型的方法是人们非常感兴趣的，并且仍然是一个高度优先的问题。[1]已经开发了几种类型的转基因方法，其中包括传统的阳离子分子介体，[2]，例如LipofeTamine2000[3]和Fu-gene 6TM，[4]病毒载体系统，[5]和“基因枪”方法。[6]随着纳米生物技术的快速发展，各种新材料，如金纳米颗粒、[7]二氧化硅纳米颗粒、[8]聚合物、[9]纳米凝胶，[10]和树枝状大分子[11]已被作为生物相容性转运体进行了研究。最近，碳纳米管--一种被充分研究的纳米材料--因其与生命系统相互作用和影响生命系统的能力而受到研究。例如，碳纳米管已被发现在聚合酶链式反应中增强DNA扩增[12]，并影响神经元的生长模式。已经报道了异硫氰酸荧光素标记的纳米管[14]的内化和编码β-半乳糖苷酶的基因[15]的纳米管输送到细胞内，没有明显的毒性作用。Kam等人。已经研究了蛋白质结合的碳纳米管通过内吞途径进入细胞的机制。[16，17]在这里，我们的发现是氨基功能化的多壁碳纳米管(NH2-MWCNTs)能够与质粒DNA相互作用，并将绿色荧光蛋白(GFP)基因传递到培养的人类细胞中。我们的数据有力地表明，碳纳米管可以被认为是一种新的载体，用于向哺乳动物细胞输送生物分子，如DNA、蛋白质和多肽。因此，这一新的系统可能在生物学和治疗方面有潜在的应用，包括疫苗和基因递送。
Exogenous-gene expression and manipulation in mammalian cells has become a mainstay of biomedical research. Consequently, improving methods for efficient gene transfer to a broad range of cell types is of great interest and remains a high priority.[1] Several classes of transfection methods have been developed, which include traditional cationic moleculemediated agents,[2] such as Lipofectamine2000 [3] and Fu-GENE 6TM,[4] viral-vector systems,[5] and the “gene gun” approach.[6] With the rapid development of nanobiotechnology, a variety of new materials, such as gold nanoparticles,[7] silica nanoparticles,[8] polymers,[9] nanogels,[10] and dendrimers [11] have been investigated as biocompatible transporters. Recently, carbon nanotube—a well-studied nanomaterial—have been investigated for their ability to interact with and affect living systems. For instance, carbon nanotubes have been found to enhance DNA amplification in PCR [12] and affect the growth pattern of neurons.[13] Pantarotto et al. have reported the internalization of fluorescein isothiocynate (FITC) labeled nanotubes [14] and nanotube delivery of the gene that encodes β-galactosidase [15] into cells, with no apparent toxic effects. Kam et al. have studied the mechanism of protein-conjugated carbon nanotube uptake into cells via the endocytic pathway.[16, 17]Here we present our finding that amino-functionalized multiwalled carbon nanotubes (NH2-MWCNTs) are able to interact with plasmid DNA and deliver the green fluorescence protein (GFP) gene into cultured human cells. Our data strongly suggest that carbon nanotubes can be considered as a new carrier for the delivery of biomolecules, such as DNA, proteins, and peptides into mammalian cells. Therefore, this novel system might have potential applications in biology and therapy, including vaccine and gene delivery.