Thiamine-regulated gene expression of Aspergillus oryzae thiA requires splicing of the intron containing a riboswitch-like domain in the 5′-UTR
Thiamine-regulated gene expression of Aspergillus oryzae thiA requires splicing of the intron containing a riboswitch-like domain in the 5′-UTR
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DOI：
10.1016/s0014-5793(03)01335-8
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发表时间：
2003-12-18
期刊：
影响因子：
3.5
通讯作者：
Hanamoto, H
中科院分区：
文献类型：
--
作者：
Kubodera, T;Watanabe, M;Hanamoto, H
外源硫胺素调节参与硫胺素合成的Aspergillus lasthiA。在其5 '-非翻译区（5'-UTR）的两个内含子中的一个包含在真菌硫胺素生物合成基因中高度保守的基序（区域A和B）。删除任何一个区域都可以解除硫胺素的抑制，硫胺素抑制内含子剪接，这表明区域A和B是有效剪接所必需的。此外，转录剪接是必不可少的thiA基因的表达。这些观察结果表明，一种新的基因表达调控机制，在丝状真菌，外源硫胺素控制内含子剪接调控基因表达。有趣的是，区域A和B构成了最近在thiA的5 '-UTR中发现的焦磷酸硫胺素结合核糖开关样结构域的一部分。(C)2003年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
Exogenous thiamine regulates Aspergillus oryzae thiA, which is involved in thiamine synthesis. One of the two introns in its 5'-untranslated region (5'-UTR) contains motifs (regions A and B) highly conserved among fungal thiamine biosynthesis genes. Deletion of either region relieved the repression by thiamine and thiamine inhibited intron splicing, suggesting that regions A and B are required for efficient splicing. Furthermore, transcript splicing was essential for thiA gene expression. These observations suggest a novel gene expression regulatory mechanism in filamentous fungi, in which exogenous thiamine controls intron splicing to regulate gene expression. Interestingly, regions A and B constitute a part of a thiamine pyrophosphate-binding riboswitch-like domain that has been quite recently found in the 5'-UTR of thiA. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.