Luminal and cancer cells in the breast show more rapid telomere shortening than myoepithelial cells and fibroblasts
Luminal and cancer cells in the breast show more rapid telomere shortening than myoepithelial cells and fibroblasts
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DOI：
10.1016/j.humpath.2008.04.005
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发表时间：
2008-11-01
期刊：
影响因子：
3.3
通讯作者：
Kaminishi, Michio
中科院分区：
文献类型：
--
作者：
Kurabayashi, Rie;Takubo, Kaiyo;Kaminishi, Michio
严重缩短、功能失调的端粒可能在癌症中常见的遗传不稳定性中发挥作用。我们分析了 30 份来自 34 至 91 岁女性的浸润性乳腺癌手术标本，并使用我们的组织定量荧光原位杂交方法估计了构成乳腺组织的 5 种不同细胞类型中的端粒长度，即端粒与着丝粒比值，以便澄清个体内部和个体之间的端粒长度变化。我们获得了 3 项新颖的发现。 (1)在相应的正常组织中，端粒长度按肌上皮细胞>正常成纤维细胞>管腔上皮细胞的顺序减小，并且每个个体内这3种细胞类型的端粒长度具有特征性。 (2) 正如预期的那样，癌细胞的端粒明显短于肌上皮细胞（P
Critically shortened, dysfunctional telomeres may play a role in the genetic instabilities commonly found in cancer. We analyzed 30 surgical specimens of invasive breast carcinoma from women aged 34 to 91 years and estimated telomere lengths as telomere-to-centromere ratio values in the 5 different cell types comprising breast tissue in order to clarify telomere length variations within and between individuals Using our tissue quantitative fluorescence in situ hybridization method. We obtained 3 novel findings. (1) In corresponding normal tissues, telomere length decreased in the order myoepithelial cells > normal-appearing fibroblasts > luminal epithelial cells, and telomere lengths were characteristic in these 3 cell types within each individual. (2) As expected, cancer cells had significantly shorter telomeres than myoepithelial cells (P