ISOLATION AND CHARACTERIZATION OF SENDAI VIRUS DI-RNAS
ISOLATION AND CHARACTERIZATION OF SENDAI VIRUS DI-RNAS
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DOI：
10.1016/0092-8674(76)90223-3
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发表时间：
1976-01-01
期刊：
影响因子：
64.5
通讯作者：
KOLAKOFSKY, D
中科院分区：
文献类型：
--
作者：
KOLAKOFSKY, D
在高密度传代时，4株仙台病毒均含有缺陷干扰(DI)颗粒。从感染了高多样性传代群体的细胞的核衣壳中分离到的RNA只由少量的非缺陷基因组长度的RNA和大量的较小的RNA组成，即DI-RNAs。这些DI-RNA在蔗糖梯度中具有不寻常的和可变的沉淀特性，但通过EM和杂交的长度测量代表了病毒基因组的独特片段。DI-RNA的一个显著特征是它们能够形成环状结构，表明DI-RNA的末端是互补的。讨论了这一发现在基因组复制机制方面的意义。
When passaged at high multicity [in embryonated chicken eggs], 4 strains of Sendai virus all showed evidence that they contained defective interfering (DI) particles. RNA isolated from nucleocapsids of cells infected with the high multiplicity passage stocks consisted of only minor amounts of nondefective genome length RNA and major amounts of smaller RNAs, the DI-RNAs. These DI-RNAs had unusual and variable sedimentation properties in sucrose gradients, but represented unique segments of the viral genome by length measurements by EM and by hybridization. A striking feature of the DI-RNAs is their ability to form circular structures, indicating that the ends of the DI-RNA are complementary. The implications of this finding in terms of the mechanism of genome replication is discussed.