Neuroblastic medulloblastoma with abundant cytoplasmic actin filaments.
Neuroblastic medulloblastoma with abundant cytoplasmic actin filaments.
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神经母细胞髓母细胞瘤，具有丰富的细胞质肌动蛋白丝。
DOI：
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发表时间：
1984
影响因子：
4.6
通讯作者：
J. Powers
中科院分区：
文献类型：
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作者：
P. Biggs;J. Powers
对具有异常形态特征的中线小脑髓母细胞瘤的常规光学显微镜检查显示出与经典髓母细胞瘤相同的特征。神经胶质原纤维酸性蛋白 (GFA)、神经丝蛋白 (NF) 和骨骼肌肌动蛋白的免疫过氧化物酶染色显示肌动蛋白的弥漫性细胞质染色，但 GFA 或 NF 没有。从超微结构上看，细胞具有许多钝的细胞质突起，具有丰富的、通常平行的 4 至 5 nm 丝。其他超微结构特征包括分散、透明和致密的核心囊泡（80 至 90 nm）、微管、稀有中间丝、多核糖体、粗面内质网和细胞连接。质膜密度和内陷偶尔与 40 至 50 nm 透明囊泡簇相关，但很少遇到，被解释为不成熟或流产的突触。钝的细胞质突起含有丰富的肌动蛋白丝、几个微管和大的透明囊泡，类似于发育中的神经细胞的生长锥。
Routine light microscopic examination of a midline cerebellar medulloblastoma with unusual morphologic features disclosed features identical to those of classic medulloblastoma. Immunoperoxidase staining for glial fibrillary acidic protein (GFA), neurofilament protein (NF), and skeletal muscle actin showed diffuse cytoplasmic staining for actin, but not for GFA or NF. Ultrastructurally, the cells had many blunt cytoplasmic processes with abundant, often parallel, 4- to 5-nm filaments. Other ultrastructural features included scattered, clear, and dense-core vesicles (80 to 90 nm), microtubules, rare intermediate filaments, polyribosomes, rough endoplasmic reticulum, and cell junctions. Plasmalemmal densities and invaginations, occasionally associated with clusters of 40- to 50-nm clear vesicles, were encountered infrequently and were interpreted as immature or abortive synapses. The blunt cytoplasmic processes containing abundant actin filaments, several microtubules, and large clear vesicles resemble growth cones of developing nerve cells.