Preliminary Results of Ex Vivo Multispectral Photoacoustic Imaging in the Management of Thyroid Cancer
Preliminary Results of Ex Vivo Multispectral Photoacoustic Imaging in the Management of Thyroid Cancer
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DOI：
10.2214/ajr.13.11433
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发表时间：
2014-06-01
影响因子：
5
通讯作者：
Rao, Navalgund A.
中科院分区：
文献类型：
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作者：
Dogra, Vikram S.;Chinni, Bhargava K.;Rao, Navalgund A.
目标。本研究的目的是验证体外多光谱光声成像是否可以用于区分恶性组织、良性结节和正常甲状腺组织。50例因甲状腺病变而行甲状腺切除术的患者参加了这项研究。对手术切除的甲状腺组织进行了多光谱光声成像，并重建了代表脱氧血红蛋白、氧合血红蛋白、脂肪和水的光学吸收的生色团图像。成像程序后，病理学家在组织病理切片上标记恶性组织、良性结节和正常区域，并获得标记的组织病理切片的数字图像。组织病理学图像与发色团图像进行配准。在发色团图像上定义了与恶性组织、良性结节和正常组织相对应的区域。结果：恶性结节和良性结节的平均脱氧血红蛋白强度差异有统计学意义(p=0.014)。恶性肿瘤组织和正常组织在脱氧血红蛋白(p=0.003)、脂质(p=0.001)和水(p<；0.0001)的平均强度上存在差异。良性结节和正常组织在氧合血红蛋白(p<；0.0001)、脂类(p<；0.0001)和水(p<；0.0001)的平均强度上存在差异。该系统对鉴别甲状腺良恶性病变的灵敏度、特异度、阳性预测值和阴性预测值分别为69.2%、96.9%、81.8%和93.9%。结论：本研究的初步结果表明，多光谱光声成像技术可用于鉴别甲状腺良恶性结节和正常甲状腺组织。
OBJECTIVE. The purpose of this study was to validate whether ex vivo multispectral photoacoustic imaging can be used to differentiate malignant tissue, benign nodules, and normal human thyroid tissue.SUBJECTS AND METHODS. Fifty patients undergoing thyroidectomy because of thyroid lesions participated in this study. Multispectral photoacoustic imaging was performed on surgically excised thyroid tissue, and chromophore images that represented optical absorption of deoxyhemoglobin, oxyhemoglobin, lipid, and water were reconstructed. After the imaging procedure, the pathologist marked malignant tissue, benign nodules, and normal regions on histopathologic slides, and digital images of the marked histopathologic slides were obtained. The histopathologic images were coregistered with chromophore images. Areas corresponding to malignant tissue, benign nodules, and normal tissue were defined on the chromophore images. Pixel values within each area were averaged to determine the mean intensities of deoxyhemoglobin, oxyhemoglobin, lipid, and water.RESULTS. There was a statistically significant difference between malignant and benign nodules with respect to mean intensity of deoxyhemoglobin (p = 0.014). There was a difference between malignant and normal tissue in mean intensity of deoxyhemoglobin (p = 0.003), lipid (p = 0.001), and water (p < 0.0001). A difference between benign nodules and normal tissue was found in mean intensity of oxyhemoglobin (p < 0.0001), lipid (p < 0.0001), and water (p < 0.0001). The sensitivity, specificity, and positive and negative predictive values of the system tested in differentiating malignant from nonmalignant thyroid tissue were 69.2%, 96.9%, 81.8%, and 93.9%.CONCLUSION. The preliminary results of this ex vivo human thyroid study suggest that multispectral photoacoustic imaging can be used to differentiate malignant and benign nodules and normal human thyroid tissue.