Using dysprosium complexes to probe the nitrogenase paramagnetic centers.
Using dysprosium complexes to probe the nitrogenase paramagnetic centers.
复制标题
使用镝络合物探测固氮酶顺磁中心。
DOI：
10.1021/bi00074a017
复制
发表时间：
1993
期刊：
影响因子：
2.9
通讯作者：
Hales,BJ
中科院分区：
文献类型：
--
作者：
Oliver,ME;Hales,BJ
MATERIALS AND METHODSMaterials and Preparations. Both component proteins of nitrogenase from A. vinelandii were isolated and purified using a variation (Hales et al., 1986) of published procedures (Burgess etal., 1980). Nitrogenase isolated from Clostridium pasteurianum (Cpl and Cp2) was generously donated by Dr. Richard Barr (Exxon Research Laboratories, Annandale, NJ). Using acetylene reduction as a monitor of activity, specific activities (in units of nanomoles of C2H2 reduced per minute per milligram of protein) for the different proteins used in this study were 1800 (Avl), 1500 (Av2), 2200 (Cpl), and 1700 (CP2).
