Astragali Radix elicits anti-inflammation via activation of MKP-1, concomitant with attenuation of p38 and Erk
Astragali Radix elicits anti-inflammation via activation of MKP-1, concomitant with attenuation of p38 and Erk
复制标题
DOI：
10.1016/j.jep.2007.09.027
复制
发表时间：
2008-01-17
影响因子：
5.4
通讯作者：
Lee, Jong-Soo
中科院分区：
文献类型：
--
作者：
Ryu, Minsook;Kim, Eun Hye;Lee, Jong-Soo
黄芪是黄芪的根，在东方医学中被广泛应用于增强免疫反应和改善血液循环，但其潜在的作用机制(S)尚不清楚。在这里，我们报道了AR通过减少iNOS、COX-2、IL-6、IL-1β和TNF-α的表达以及减少一氧化氮(NO)的产生而对酵母多糖气囊小鼠起到抗炎作用。在类似的方式，AR减少IL-6，诱导型一氧化氮合酶和环氧合酶-2的表达在脂多糖(脂多糖)处理的RAW 264.7细胞。我们进一步证明，AR减弱了脂多糖处理的RAW 264.7细胞中p38和ERK1/2的活性，并刺激了丝裂原激活的蛋白激酶磷酸酶-1(MKP-1)。此外，AR干扰了核转录因子kappaB向核的移位，从而导致依赖于核因子kappaB的转录抑制。综上所述，这些数据表明，AR的抗炎作用是通过依赖于MKP-1的p38和ERK1/2失活以及抑制NF-kappa B介导的转录来实现的。这些结果表明，AR草药具有潜在的抗炎活性。(C)2007爱思唯尔爱尔兰有限公司。保留所有权利。
Although Astragali Radix (Astragalus, AR), the root of Astragalus membranaceus (Fisch) Bunge, is widely used in oriental medicine for tonifying the immune response and improving circulation, the underlying mechanism(s) by which these effects are induced remains unclear. Here, we report that AR displays anti-inflammatory effects in zymosan air-pouch mice by reducing the expression of iNOS, COX-2, IL-6, IL-1 beta and TNF-alpha and by decreasing the production of nitric oxide (NO). In a similar manner, AR reduces the expression of IL-6, iNOS, and COX-2 in lipopolysaccharide (LPS)-treated Raw 264.7 cells. We further demonstrate that AR attenuates the activity of p38 and Erk1/2 and stimulates mitogen-activated protein kinase phosphatase-1 (MKP-1) in LPS-treated Raw 264.7 cells. Additionally, AR interferes with the translocation of NF kappa B to the nucleus, subsequently resulting in NF kappa B-dependent transcriptional repression. Taken together, these data reveal that AR has an anti-inflammatory effect that is mediated by the MKP-1-dependent inactivation of p38 and Erk1/2 and inhibition of NF kappa B-mediated transcription. These results imply that the AR herb has a potential anti-inflammatory activity. (C) 2007 Elsevier Ireland Ltd. All rights reserved.