Modulation of extracellular matrix synthesis and alkaline phosphatase activity of periodontal ligament cells by mechanical stress
Modulation of extracellular matrix synthesis and alkaline phosphatase activity of periodontal ligament cells by mechanical stress
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DOI：
10.1111/j.1600-0765.2004.00782.x
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发表时间：
2005-04-01
影响因子：
3.5
通讯作者：
Soma, K
中科院分区：
文献类型：
--
作者：
Ozaki, S;Kaneko, S;Soma, K
背景：据报道，咬合功能的丧失可引起牙周韧带的萎缩性变化。这可能是机械应力引起牙周韧带的生物反应。然而，关于不同强度的机械应力与牙周膜细胞活性如细胞外基质（ECM）合成之间关系的研究报道很少。目的：本研究旨在阐明机械应力对ⅰ型胶原蛋白和decorin基因mRNA表达水平以及碱性磷酸酶（ALP）活性的影响，以响应不同程度的机械应力。方法：牛牙周韧带细胞在柔性底培养板上培养，置于BioFlex加载台（TM）上。在6个拉伸水平（底部表面积增加0.2、1.0、2.0、3.0、10%、18%）下，以6 cycles/min（开、关各5 s）拉伸48 It。我们用定量逆转录聚合酶链反应（RT-PCR）检测了I型胶原和decorin基因的mRNA表达水平，以及循环机械拉伸牙周韧带细胞培养中ALP的活性。结果：低强度机械拉应力诱导牙周韧带细胞I型胶原和decorin mRNA表达增加，但未改变ALP活性。高强度机械拉应力诱导ⅰ型胶原和decorin mRNA表达增加，ALP活性降低。结论：不同强度的拉力对牙周膜细胞的影响不同，表明机械应力在牙周膜的重塑和功能调节中起重要作用。
Background: Loss of occlusal function has been reported to induce atrophic changes in the periodontal ligament. It is likely that mechanical stress triggers the biological response of periodontal ligament. However, there have been few reports studying the correlation between mechanical stress of varying magnitude and periodontal ligament cell activities such as extracellular matrix (ECM) synthesis.Objective: The objective of this study is to clarify the influence of the mechanical stress on changes in mRNA expression levels of type I collagen and decorin genes, as well as alkaline phosphatase (ALP) activity in response to mechanical stress of varying magnitude.Methods: Bovine periodontal ligament cells were cultured on flexible-bottomed culture plates and placed on the BioFlex Loading Stations(TM). Cells were elongated at 6 cycles/min (5 s on and 5 s off) at each of six levels of stretch (0.2, 1.0, 2.0, 3.0, 10, 18% increase in the surface area of the bottom) for 48 It. We measured mRNA expression levels of type I collagen and decorin genes using quantitative reverse transcription-polymerase chain reaction (RT-PCR), and ALP activity in periodontal ligament cell culture under cyclic mechanical stretching.Results: Mechanical tensional stress of low magnitude induced the increase of both type I collagen and decorin mRNA expression without changing ALP activity in periodontal ligament cells. Mechanical tensional stress of high magnitude induced the increase of type I collagen and decorin mRNA expression while decreasing ALP activity.Conclusion: These results suggest that different magnitude of tensional force induces different responses from periodontal ligament cells, and that mechanical stress plays an important role in remodeling and functional regulation of periodontal ligament.