Viraemic frequencies and seroprevalence of non-primate hepacivirus and equine pegiviruses in horses and other mammalian species
Viraemic frequencies and seroprevalence of non-primate hepacivirus and equine pegiviruses in horses and other mammalian species
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DOI：
10.1099/vir.0.065094-0
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发表时间：
2014-08-01
影响因子：
3.8
通讯作者：
Simmonds, Peter
中科院分区：
文献类型：
--
作者：
Lyons, Sinead;Kapoor, Amit;Simmonds, Peter
非灵长类动物肝炎病毒（NPHV）、马拟吉病毒（EPgV）和泰勒氏病相关病毒（TDAV）分别是黄病毒科hepacivirus和pegivirus两属的新发现成员，包括人类丙型肝炎病毒（HCV）和人类拟吉病毒（HPgV）。为了研究它们的流行病学、持续性和感染的临床特征，对大群马和其他哺乳动物进行了NPHV、EPgV和TDAV病毒血症筛查，并通过NPHV和EPgV特异性抗体的血清学检测来筛查过去的暴露情况。对328匹马进行NS3抗体和核心抗体筛选，其中43%检测到NPHV抗体，其中3匹经PCR检测呈病毒型。与同一农场的一匹病毒马同住的五匹马全部呈血清阳性，同一农场的一条狗也是如此。除了这一个例外，所有其他物种的NPHV抗体和病毒血症均为阴性：驴（n=100），狗（n=112），猫（n=131），非人灵长类动物（n=164）和人类（n=362）。66.5%的马检测到EPgV NS3抗体，包括12匹EPgV病毒血症马中的10匹。所有驴标本EPgV抗体和RNA均为阴性。所有马和驴样本TDAV RNA均为阴性。通过比较有和没有肝病的马的病毒血症频率，没有证据支持活动性NPHV和EPgV感染与肝病之间的关联。该研究表明，NPHV和EPgV感染在研究的马种群中广泛存在，并且是地方性的，并证实NPHV和潜在的EPgV在人类中比HCV和HPgV感染具有更高的病毒清除率。
Non-primate hepacivirus (NPHV), equine pegivirus (EPgV) and Theiler's disease associated virus (TDAV) are newly discovered members of two genera in the Flaviviridae family, Hepacivirus and Pegivirus respectively, that include human hepatitis C virus (HCV) and human pegivirus (HPgV). To investigate their epidemiology, persistence and clinical features of infection, large cohorts of horses and other mammalian species were screened for NPHV, EPgV and TDAV viraemia and for past exposure through serological assays for NPHV and EPgV-specific antibodies. NPHV antibodies were detected in 43% of 328 horses screened for antibodies to NS3 and core antibodies, of which three were viraemic by PCR. All five horses that were stablemates of a viraemic horse were seropositive, as was a dog on the same farm. With this single exception, all other species were negative for NPHV antibodies and viraemia: donkeys (n=100), dogs (n=112), cats (n=131), non-human primates (n=164) and humans (n=362). EPgV antibodies to NS3 were detected in 66.5% of horses, including 10 of the 12 horses that had EPgV viraemia. All donkey samples were negative for EPgV antibody and RNA. All horse and donkey samples were negative for TDAV RNA. By comparing viraemia frequencies in horses with and without liver disease, no evidence was obtained that supported an association between active NPHV and EPgV infections with hepatopathy. The study demonstrates that NPHV and EPgV infections are widespread and enzootic in the study horse population and confirms that NPHV and potentially EPgV have higher frequencies of viral clearance than HCV and HPgV infections in humans.