Underexpression of a novel gene, Dia2, impairs the transition of Dictyostelium cells from growth to differentiation
Underexpression of a novel gene, Dia2, impairs the transition of Dictyostelium cells from growth to differentiation
复制标题
DOI：
10.1006/bbrc.1998.9461
复制
发表时间：
1998-11-09
影响因子：
3.1
通讯作者：
Maeda, Y
中科院分区：
文献类型：
--
作者：
Chae, SC;Inazu, Y;Maeda, Y
在盘基网柄藻Ax-2细胞中，已经指定了细胞周期中的一个特定点（PS点），从该点它们响应于饥饿而开始分化。使用同步化的Ax-2细胞和差异显示方法，分离到一个新的基因（分化相关基因2; dia 2），作为Ax-2细胞从生长向分化转变过程中特异性表达的基因之一。dia 2基因编码富含赖氨酸和亮氨酸的蛋白质，预测分子量为16.9 kDa。北方印迹分析表明，0.7 kb的dia 2 mRNA在PS点前饥饿的分化细胞中积累，而在植物生长细胞中没有可检测的表达。反义介导的dia 2基因失活极大地抑制了分化的进展，可能是通过减少cAMP受体1（car 1）的表达。因此，DIA 2的表达被认为在分化的起始中具有重要作用，与cAMP信号系统密切相关。(C)北京：科学出版社.
In Dictyostelium discoideum Ax-2 cells, a specific point (PS-point) in the cell cycle from which they initiate differentiation in response to starvation has been specified. Using synchronized Ax-2 cells and the differential display method, a novel gene (differentiation-associated gene 2; dia2) was isolated as one of the genes expressed specifically during the shift of Ax-2 cells from growth to differentiation. The dia2 gene codes a lysine- and leucine-rich protein with a predicted molecular mass of 16.9 kDa. Northern blot analysis has shown that the dia2 mRNA, of 0.7 kb, accumulates in differentiating cells starved just before the PS-point, while there is no detectable expression in vegetatively growing cells. Antisense-mediated gene inactivation of dia2 greatly inhibited the progress of differentiation, presumably through the reduced expression of cAMP receptor 1 (car1). Thus, the DIA2 expression was suggested to have an essential role in the initiation of differentiation, closely relating to the cAMP signaling system. (C) 1998 Academic Press.