Human intestinal epithelial cells exhibit a cellular response indicating a potential toxicity upon exposure to hematite nanoparticles
Human intestinal epithelial cells exhibit a cellular response indicating a potential toxicity upon exposure to hematite nanoparticles
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DOI：
10.1007/s10565-012-9229-7
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发表时间：
2012-10-01
影响因子：
6.1
通讯作者：
Capco, David G.
中科院分区：
文献类型：
--
作者：
Kalive, Madhavi;Zhang, Wen;Capco, David G.
本研究检测了不同大小的纳米颗粒对肠上皮细胞潜在细胞毒性的影响。三种尺寸的赤铁矿纳米颗粒用于研究，浓度为10 ppm：17、53和100 nm。结果表明，在测试的赤铁矿纳米颗粒中，17 nm对上皮完整性的毒性大于53或100 nm。此外，上皮完整性受到上皮结构（如顶端微绒毛）破坏和细胞-细胞连接破坏的影响，导致跨上皮电阻测量值（TEER）降低。TEER的下降是由粘附连接的破坏而不是由细胞死亡引起的，如通过免疫细胞化学和通过使用细胞活力测定所确定的。上皮完整性也受到影响，在分子水平上显示的差异表达的基因相关的细胞连接的维护，这是由微阵列分析评估。总之，17-和100-nm的赤铁矿纳米颗粒引起上皮细胞的显着结构变化，但不是53 nm的纳米颗粒。此外，不同大小的赤铁矿纳米颗粒在细胞水平和遗传水平上都有不同的影响。
This study examined the effects of different-sized nanoparticles on potential cytotoxicity in intestinal epithelia. Three sizes of hematite nanoparticles were used for the study at a 10 ppm concentration: 17, 53, and, 100 nm. Results indicate that, of the hematite nanoparticles tested, 17 nm was more toxic to the epithelial integrity than 53 or 100 nm. In addition, the epithelial integrity was affected by disruption of epithelial structures such as apical microvilli, and by disruption of the cell-cell junctions leading to reduction in transepithelial electrical resistance measurements (TEER). The drop in TEER was caused by disruption of the adhering junctions not by cell death, as determined by immunocytochemistry, and by using a cell viability assay. Epithelial integrity was also affected at the molecular level as shown by differential expression of genes related to cell junction maintenance, which was assessed by microarray analysis. In conclusion, the 17- and 100-nm hematite nanoparticles caused significant structural changes in the epithelium but not the 53 nm nanoparticles. Also, different-sized hematite nanoparticles each had different effects both at the cellular level and genetic level.