The luminance ratio of autofluorescence in a xenograft mouse model is stable through tumor growth stages.
The luminance ratio of autofluorescence in a xenograft mouse model is stable through tumor growth stages.
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DOI：
10.1002/cre2.126
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发表时间：
2018-10
影响因子：
1.8
通讯作者：
Sumitomo S
中科院分区：
文献类型：
--
作者：
Sumi S;Umemura N;Adachi M;Ohta T;Naganawa K;Kawaki H;Takayama E;Kondoh N;Sumitomo S
本研究旨在探讨自体荧光成像在口腔癌不同生长阶段的应用价值，以协助检测肿瘤。将人口腔鳞状细胞癌细胞系HSC-3皮下接种到裸鼠中，建立异种移植小鼠模型。用自体荧光成像方法（Illumiscan®）使用亮度比进行肿瘤成像，亮度比定义为肿瘤部位的亮度相对于正常皮肤组织的亮度，标准化为1.0的值。在接种后连续观察该亮度比。收集肿瘤和正常皮肤组织，并检查黄素腺嘌呤二核苷酸和烟酰胺腺嘌呤二核苷酸浓度的差异。肿瘤部位的亮度比为0.85 ± 0.05，即使肿瘤增殖和扩大，随着时间的推移，该比值也没有显著变化。肿瘤组织中黄素腺嘌呤二核苷酸和烟酰胺腺嘌呤二核苷酸含量明显低于正常皮肤组织。亮度比低于0.90表示肿瘤的高可能性，与肿瘤生长阶段无关。然而，该临界值是使用异种移植小鼠模型确定的，因此在用于临床诊断之前需要进一步验证。
The aim of this research was to investigate the value of autofluorescence imaging of oral cancer across different stages of tumor growth, to assist in detecting tumors. A xenograft mouse model was created with human oral squamous cell carcinoma cell line HSC‐3 being subcutaneously inoculated into nude mice. Tumor imaging was performed with an autofluorescence imaging method (Illumiscan®) using the luminance ratio, which was defined as the luminance of the tumor site over the luminance of normal skin tissue normalized to a value of 1.0. This luminance ratio was continuously observed postinoculation. Tumor and normal skin tissues were harvested, and differences in the concentrations of flavin adenine dinucleotide and nicotinamide adenine dinucleotide were examined. The luminance ratio of the tumor sites was 0.85 ± 0.05, and there was no significant change in the ratio over time, even if the tumor proliferated and expanded. Furthermore, flavin adenine dinucleotide and nicotinamide adenine dinucleotide were significantly lower in tumor tissue than in normal skin tissue. A luminance ratio under 0.90 indicates a high possibility of tumor, irrespective of the tumor growth stage. However, this cutoff value was determined using a xenograft mouse model and therefore requires further validation before being used in clinical diagnosis.