A phage-displayed peptide can inhibit infection by white spot syndrome virus of shrimp.
A phage-displayed peptide can inhibit infection by white spot syndrome virus of shrimp.
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DOI：
10.1099/vir.0.19001-0
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发表时间：
2003-09
期刊：
影响因子：
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通讯作者：
Guohua Yi;J. Qian;Zhimin Wang;Y. Qi
中科院分区：
文献类型：
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作者：
Guohua Yi;J. Qian;Zhimin Wang;Y. Qi
由白色斑点综合征病毒（WSSV）引起的白色斑点病给世界各地的对虾养殖业造成了毁灭性的损失，目前还没有有效的治疗方法。本研究的目的是选择一种可能能够预防WSSV感染的噬菌体展示肽。构建了10-mer噬菌体展示肽库（滴度7.2 × 10（7）），并针对固定化WSSV进行筛选。评估所选肽的特异性和抑制病毒感染的效率。在与WSSV特异性结合的四种肽中，命名为2 E6的肽具有高特异性并阻断病毒感染，其中可能的病毒抑制关键基序是VAVNNSY。结果表明，肽2 E6具有作为抗病毒肽药物开发的潜力。
White spot disease, caused by white spot syndrome virus (WSSV), results in devastating losses to the shrimp farming industry around the world, and no effective treatments have been found. Control focuses on exclusion of the virus from culture ponds but, once introduced, spread is often rapid and uncontrollable. The purpose of this study was to select a phage-displayed peptide that might be able to prevent WSSV infection. A 10-mer phage display peptide library (titre 7.2 x 10(7)) was constructed and screened against immobilized WSSV. Selected peptides were assessed for specificity and efficiency of inhibition of virus infection. Of four peptides that specifically bound to WSSV one, designated 2E6, had a high specificity and blocked virus infection, with the possible critical motif for virus inhibition being VAVNNSY. The results suggest that peptide 2E6 has potential for exploitation as an antiviral peptide drug.