Effects of long-chain fatty acids on cytosolic triacylglycerol accumulation and lipid droplet formation in primary cultured bovine mammary epithelial cells.
Effects of long-chain fatty acids on cytosolic triacylglycerol accumulation and lipid droplet formation in primary cultured bovine mammary epithelial cells.
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DOI：
10.3168/jds.s0022-0302(04)73377-9
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发表时间：
2004-08
影响因子：
3.5
通讯作者：
T. Yonezawa;S. Yonekura;Y. Kobayashi;A. Hagino;K. Katoh;Y. Obara
中科院分区：
文献类型：
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作者：
T. Yonezawa;S. Yonekura;Y. Kobayashi;A. Hagino;K. Katoh;Y. Obara
乳腺上皮细胞最近已被证明表达和分泌瘦素到牛奶和积累三酰甘油（TAG）在胞质溶胶中。我们研究了游离脂肪酸对细胞溶质TAG积累的影响，此外，培养基沐浴牛乳腺上皮细胞（bMEC）。饱和脂肪酸（棕榈酸和硬脂酸）和不饱和脂肪酸（油酸和亚油酸）均以浓度依赖性方式刺激TAG在50至400 μ M范围内的积累，并刺激参与长链脂肪酸摄取和分泌的CD 36 mRNA表达。然而，瘦素mRNA的表达和脂滴的形成显着增加，只有通过添加不饱和，但不饱和，脂肪酸。有趣的是，这两种类型的脂肪酸刺激caseas 1-酪蛋白mRNA的表达。这些数据表明，瘦素的表达与液滴形成有关，而CD 36与细胞溶质TAG积累有关，并且脂肪酸或细胞溶质TAG积累也具有加速bMEC分化的作用，如酪蛋白合成所示。
Mammary epithelial cells have recently been shown to express and secrete leptin into milk and to accumulate triacylglycerol (TAG) in cytosol. We examined the effects on the accumulation of cytosolic TAG of free fatty acid addition to the medium bathing bovine mammary epithelial cells (bMEC). Both saturated (palmitic and stearic) and unsaturated (oleic and linoleic) fatty acids stimulated the accumulation of TAG in a concentration-dependent manner from 50 to 400 microM and the expression of mRNA expression for CD36, which is involved in the uptake and secretion of long-chain fatty acids. However, leptin mRNA expression and lipid droplet formation were significantly increased only by the addition of unsaturated, but not saturated, fatty acids. Interestingly, both types of fatty acids stimulated alphas1-casein mRNA expression. These data suggest that the expression of leptin is related to droplet formation, whereas CD36 is related to cytosolic TAG accumulation, and that fatty acids or cytosolic TAG accumulation also have a role to accelerate differentiation of bMEC as shown by casein synthesis.