Shift of whispering-gallery modes in microspheres by protein adsorption
Shift of whispering-gallery modes in microspheres by protein adsorption
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DOI：
10.1364/ol.28.000272
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发表时间：
2003-02-15
期刊：
影响因子：
3.6
通讯作者：
Vollmer, F
中科院分区：
文献类型：
--
作者：
Arnold, S;Khoshsima, M;Vollmer, F
利用微扰理论描述了基于微球中伴随蛋白质吸附的窃窃廊模式转移的生物传感器。对于随机空间吸附，理论预测位移应与微球半径R成反比，与蛋白质表面密度和过量极化率成正比。测量结果与理论一致，并且这种对应关系使估计单个蛋白质所占的平均表面积成为可能。这些结果与牛血清白蛋白的结晶学数据一致。发现单个蛋白质吸附的理论位移对靶区非常敏感，最敏感区域的吸附变化为1/R-5/2。预测单个蛋白质或病毒颗粒检测的特定参数。(C) 2003年美国光学学会。
Biosensors based on the shift of whispering-gallery modes in microspheres accompanying protein adsorption are described by use of a perturbation theory. For random spatial adsorption, theory predicts that the shift should be inversely proportional to microsphere radius R and proportional to protein surface density and excess polarizability. Measurements are found to be consistent with the theory, and the correspondence enables the average surface area occupied by a single protein to be estimated. These results are consistent with crystallographic data for bovine serum albumin. The theoretical shift for adsorption of a single protein is found to be extremely sensitive to the target region, with adsorption in the most sensitive region varying as 1/R-5/2. Specific parameters for single protein or virus particle detection are predicted. (C) 2003 Optical Society of America.